Zenix®-C SEC phase, developed based on innovative surface coating technology, comprises of uniform, hydrophilic, and neutral nanometer thick film chemically bonded to high purity and mechanically stabilized silica. Zenix®-C, uses lay-down monolayer on porous silica as oppose to Zenix of stand-up monolayer (as shown below), offers ideal phase chemistry for hydrophobic samples like insulin, membrane protein monoclonal antibodies derivatized with polymer branches (polypeptide or PEG) separation. The 3µm based packing material allows for high resolution and performance separation.
Stationary Phase Structure Illustration and Comparison
Phase structure difference: a monolayer stands up on the silica surface for SRT® and Zenix®, and a monolayer lays down on the silica surface for SRT®-C and Zenix®-C.
"Tough samples" such as hydrophobic proteins like insulin, membrane protein monoclonal antibodies derivatized with polymer branches, e.g. polypeptide, PEG.
Zenix-C Technical Specifications
Phase
Zenix-C SEC-80
Zenix-C SEC-100
Zenix-C SEC-150
Zenix-C SEC-300
Material
Neutral, hydrophilic film bonded silica
Particle size
3 µm
Pore size
~ 80 Å
~ 100 Å
~ 150 Å
~ 300 Å
Protein MW range (native)
100 - 50,000
100 - 66,000
500 - 150,000
5,000 - 1,250,000
pH stability
2.5 – 7.5 (up to pH 8.5 can be tolerated temporarily*)
Backpressure (for a 7.8×300 mm)
~ 1,885 psi
~ 1,500 psi
~ 1,375 psi
~ 1,100 psi
Backpressure (for a 4.6×300 mm)
~ 1,450 psi
~ 1,400 psi
~ 1,250 psi
~ 1,000 psi
Maximum backpressure
~ 4,500 psi
~ 4,500 psi
~ 4,500 psi
~ 3,500 psi
Salt concentration range
20 mM – 500 mM
Maximum temperature
~4 – 40°C
Mobile phase compatibility
Aqueous and organic
Ideal for separation and analysis of hydrophobic proteins, and monoclonal antibodies derivatized with polymer branches such as polypeptide or PEG
Particle size of 3 µm
Selection of pore size: 100, 150 and 300 Å
Highest separation efficiency and resolution
High capacity
High lot-to-lot reproducibility
High protein recovery with intact biological activity
Negligible non-specific interactions
Separation of a protein mixture by Zenix-C SEC-300 column.
[1] Guard column [2] Sepax Protein standard for size exclusion chromatography and used for general correlation of elution time vs. protein MW [3] One-piece PEEK coupler, recommended to connect columns in series. [4] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [5] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [6] Includes one ZenixTM 300 Å, and one ZenixTM-C 300 Å, columns (7.8 x 300mm). [7] Includes one ZenixTM 300 Å, and one ZenixTM-C 300 Å, columns (4.6 x 300mm). [8] 0.5 µm PEEK frit for column with particle size 3 µm or less [9] For Precolumn Filter (0.5 µm) 5 units/pk
[1] Guard column [2] Column packed with PEEK tubing. [3] Sepax Protein standard for size exclusion chromatography and used for general correlation of elution time vs. protein MW [4] One-piece PEEK coupler, recommended to connect columns in series. [5] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [6] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [7] Includes one ZenixTM 300 Å, and one ZenixTM-C 300 Å, columns (7.8 x 300mm). [8] Includes one ZenixTM 300 Å, and one ZenixTM-C 300 Å, columns (4.6 x 300mm). [9] 0.5 µm PEEK frit for column with particle size 3 µm or less [10] Intended for use with column IDs ≥ 21.2 mm [11] For Precolumn Filter (0.5 µm) 5 units/pk [12] SS Refill Frits for 102020-21200, 5 units/pk
[1] Guard column [2] Column packed with PEEK tubing. [3] Sepax Protein standard for size exclusion chromatography and used for general correlation of elution time vs. protein MW [4] One-piece PEEK coupler, recommended to connect columns in series. [5] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [6] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [7] Includes one ZenixTM 300 Å, and one ZenixTM-C 300 Å, columns (7.8 x 300mm). [8] Includes one ZenixTM 300 Å, and one ZenixTM-C 300 Å, columns (4.6 x 300mm). [9] 0.5 µm PEEK frit for column with particle size 3 µm or less [10] Intended for use with column IDs ≥ 21.2 mm [11] For Precolumn Filter (0.5 µm) 5 units/pk [12] SS Refill Frits for 102020-21200, 5 units/pk
[1] Guard column [2] Column packed with PEEK tubing. [3] Sepax Protein standard for size exclusion chromatography and used for general correlation of elution time vs. protein MW [4] One-piece PEEK coupler, recommended to connect columns in series. [5] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [6] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [7] Includes one ZenixTM 300 Å, and one ZenixTM-C 300 Å, columns (7.8 x 300mm). [8] Includes one ZenixTM 300 Å, and one ZenixTM-C 300 Å, columns (4.6 x 300mm). [9] 0.5 µm PEEK frit for column with particle size 3 µm or less [10] Intended for use with column IDs ≥ 21.2 mm [11] For Precolumn Filter (0.5 µm) 5 units/pk [12] SS Refill Frits for 102020-21200, 5 units/pk
