Polar MC-IMAC Excel resins are metal affinity chromatography resins for the purification of recombinant proteins with a histidine (His) tag, which are widely used in the biotech industry. The resins are composed of hydrophilic polymethacrylate beads with high physical and chemical stability. The resins have a particle size of 30 µm or 60 µm with a pore size of 800 Å. The resin surface is highly hydrophilic, which minimizes non-specific interaction with biological analytes. On the hydrophilic surface, carboxylic acid groups which can chelate multivalent metal ions are chemically attached via a proprietary linker optimized for bio-separations
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A customer using Sepax Polar MC Ni-MC60 IMAC resin for their protein projects shared the following:
“The Sepax IMAC resin offers better flow rates when compared to traditional agarose based resins. The Sepax base bead is a rigid PMA which allows for higher flow rates, a packed bed that is less likely to compress, and in return, a higher column bed height can be achieved.
Since the Sepax IMAC resin is EDTA resistant, I am able to use the column multiple times before CIP and additionally, my CIP protocol is shortened since the column does not need to be recharged. Removing the need for regeneration has not only saved me hours of instrument time, but I also now have less buffer to prepare!”
Aqueous solutions, a mixture of water and acetonitrile, acetone, or methanol. Typical buffers: phosphate, Tris, and acetate, also compatible with Guanidine HCl 6 M, Urea 8 M, EDTA 0.1 M, DTT 10 mM
Linear Flow Rate
Up to 1800 cm/hour
Bulk Resin
Resin
Particle Size
Pore Size
Part Number
Volume
Add To Cart
Polar MC30-CA Excel
30 µm
800 Å
270530800
Polar MC60-CA Excel
60 µm
800 Å
270560800
Polar MC30-Ni Excel
30 µm
800 Å
270630800
Polar MC60-Ni Excel
60 µm
800 Å
270660800
Part Number
End Fitting
Column Size (mm)
Bed Height (cm)
Volume (mL)
Quote
202000-0615-AF
A/F
6.6x150
4-12
1.4-4.1
202000-0615-FF
F/F
6.6x150
12
4.1
202000-0625-AA
A/A
6.6x250
6-22
2.1-7.5
202000-0625-AF
A/F
6.6x250
14-22
4.8-7.5
202000-0640-AA
A/A
6.6x400
21-37
7.2-12.7
202000-1015-AF
A/F
10.0x150
4-12
3.1-9.4
202000-1025-AA
A/A
10.0x250
6-22
3.1-17.3
202000-1025-AF
A/F
10.0x250
14-22
19.63
202000-1515-AF
A/F
15.0x150
4-12
7.1-21.2
202000-1525-AA
A/A
15.0x250
6-22
10.6-38.9
202000-1525-AF
A/F
15.0x250
14-22
24.7-38.9
202000-2515-AF
A/F
25.0x150
4-12
19.6-58.9
202000-2525-AA
A/A
25.0x250
6-22
29.4-108.0
202000-2525-AF
A/F
25.0x250
14-22
68.7-108.0
202000-3540-AF
A/F
35.0x400
29-37
279.0-355.9
202000-5015-AF
A/F
50.0x150
4-12
77.5-235.0
202000-5025-AA
A/A
50.0x250
5.8-22.0
120.2-440.7
202000-5040-AA
A/A
50.0x400
21-37
420.5-741.0
202000-5040-AF
A/F
50.0x400
29-37
580.7-741.0
202000-5050-AA
A/A
50.0x500
31-47
620.8-941.2
202000-5050-AF
A/F
50.0x500
39-47
781.0-941.2
AF: One fixed endpiece and one adjustable endpiece
AA: Two adjustable endpieces
FF: Non-adjustable with two fixed endpieces
