Sepax Monomix MC-Boronate affinity resins are designed to purify and analyze cis-diol containing compounds, such as glycoproteins,
nucleic acids and sugars. The base beads are monosized, composed of hydrophilic polymethacrylate (PMA) of high physical and chemical
stability, with m-Aminophenyl boronate covalently linked using a proprietary hydrophilic linker. This highly hydrophilic resin surface
allows for minimized non-specific bindings with biological samples. The resin is currently offered with particle sizes of 30 and 60 μm
and pore size of 1000 Å. The exclusion limit of dextran is approximately 2 x 106, and the exclusion limit of globular proteins is
approximately 1 x 107, suitable for industrial purification. In addition, Sepax can provide high-resolution analytical chromatography
columns under the product brand of Monomix MC-Boronate with a particle size of 10 μm and a pore size of 500 Å and 1000 Å.
Characteristics
High binding capacity and excellent biocompatibility
Capable of withstanding high pressure and high flow rate due to rigid base bead material
High separation resolution, efficiency and recovery
High lot-to-lot consistency
Easy to scale up
Highly hydrophilic surface with minimal non-specific binding
Small volume change under normal packing conditions
Other cis-1,2-diols, cis-1,2-hydroxy acids and cis-1,2-hydroxylamine containing samples
polyphenols (catechols)
Sepax affinity resins are especially suited to the purification of glycoproteins, or the removal of glycoprotein and carbohydrate
impurities from non-glycosylated molecules. In addition, Sepax can provide high-resolution analytical chromatography columns under
the product brand of Monomix MC-Boronate with a particle size of 10 μm and a pore size of 500 Å and 1000 Å.
