MabPurix A Series: Agarose-based Protein A Affinity Chromatography Resins
MabPurix A45 and MabPurix A65 affinity resins are based on spherical, highly cross-linked agarose beads with a narrow
size distribution and high mechanical stability. The agarose base beads are covalently bonded with a base resistant
recombinant Protein A through a proprietary method. MabPurix A affinity resins are specifically designed for purification
applications of monoclonal antibodies and recombinant protein biomolecules containing Fc domains. Compared to other
conventional agarose affinity resins, MabPurix A resins offer a better pressure resistance.
MabPurix P Series: Polymer-based Protein A Affinity Chromatography Resins
MabPurix P30 and MabPurix P45 affinity resins are made from monodispersed porous polymethacrylate based beads
with average particle sizes of 30 µm and 45 µm respectively. The base beads are first treated with proprietary coating
technology to provide hydrophilicity and bioinertness to avoid non-specific binding, and then are covalently bonded with
a base resistant recombinant Protein A through a proprietary method. MabPurix P affinity resins are specifically designed
for purification applications of monoclonal antibodies and recombinant protein biomolecules containing Fc domains.
Specification
Resin
MabPurix P30
MabPurix P45
MabPurix A45
MabPurix A65
Matrix
PMA
PMA
Agarose
Agarose
Ligand
30 µm
45 µm
45 µm
65 µm
Ligand
rProtein A alkaline tolerant
rProtein A alkaline tolerant
rProtein A alkaline tolerant
rProtein A alkaline tolerant
DBC
>50 hlgG/mL resin (4 min residence time)
>50 hlgG/mL resin (4 min residence time)
>70 hlgG/mL resin (4 min residence time)
>50 hlgG/mL resin (4 min residence time)
Maximum Back Pressure
1 MPa
1 MPa
0.3 MPa
0.3 MPa
pH Stability
1-13
1-13
3-12
3-12
0.5 M NaOH Stability
Shaking 25 h, DBC >80%
Shaking 25 h, DBC >80%
Shaking 25 h, DBC >70%
Shaking 25 h, DBC >70%
CIP
Up to 0.5 M NaOH
Up to 0.5 M NaOH
Up to 0.5 M NaOH
Up to 0.5 M NaOH
High loading capacity (MabPurix A45)
Protein A loss and HCP were well controlled at very low level
Fast antibody binding, high efficiency, enable short residence time operation/ fast sample loading
