Sepax Technologies, Inc.

Sepax HP-C18 Columns

Description

Packing

Characteristics

Application

Order Info

Downloads

HP-C18 uses full coverage bonded silica packing, which provides high stability. Compatible with 100% aqueous mobile phase and suitable for separations of acidic, neutral and basic organic compounds, as well as pharmaceuticals and peptides. ODS monolayer formed by special bonding chemistry does not collapse in pure aqueous solution.

Silica:	Spherical, high purity (<10 ppm metals)
Pore size:	120 Å
Particle size:	3, 5, and 10 µm
Pore volume:	1.0 ml/g
Surface area:	300 m²/g
Phase structure:	Monomeric and fully endcapped
% Carbon:	17%

Silica:	Spherical, high purity (<10 ppm metals)
Pore size:	200 Å
Particle size:	3 and 5 µm
Pore volume:	1.0 ml/g
Surface area:	200 m²/g
Phase structure:	Monomeric and fully endcapped
% Carbon:	10%

Highly controlled chemistry of monolayer formation and end-capping
Extremely high column-to-column reproducibility
High selectivity and efficiency for separations
Compatible with 100% aqueous mobile phase
Suitable for separations of acidic, neutral and basic organic compounds, as well as pharmaceuticals, peptides, and others

Stability

Sepax HP-C18 uses full coverage bonded silica packing, which provides the exceptionally high stability. Figure 1 shows the highly reproducible retention time for three standard compounds: aniline, anisole and toluene after 13,000 column volume runs in a mobile phase of 85% methanol and 15% water. Such high stability allows Sepax HP-C18 extremely suitable for validation of various analytes.

Reproducibility

Figure shown below is a real application of Sepax HP-C18, showing fast separation of 8 pharmaceutical molecules by a 3 µm, 4.6×50mm short column. After 1800 column volume of "0.1% TFA and CH₃CN mobile phase" was pumped, the column still shows almost identical performance!

(Courtesy of Miyako Kawakatsu, M&S Instruments Inc.)

Selectivity

Sepax HP-C18 has better selectivity and resolution for organometallic complex isomers compared to other competitors' C18 phases.

(Courtesy of Dr. John Lin of Avantix Laboratories)

Conditions
Column selection:	1. Phenomenex, Synergi, Polar-RP, 4 µm, 50×2.0 mm 2. Keystone, AQ C18, 3 µm, 50×2.0 mm 3. MetaChem, Polaris-C18, 3 µm, 50×2.1 mm 4. Sepax HP C18, 3 µm, 50×2.1 mm
Mobile phase:	A: 0.1% TFA in water with 10 mM ammonium formate B: 0.1% TFA in methanol with 10 mM ammonium formate
Gradient elution
Flow rate:	0.5 mL/min
Injection volume:	10 µL
Temperature:	25 °C
Instrument:	API 4000 LC-MS/MS system
Compounds:	Two organometallic complex isomers: M40403 and M40531 (The molecular structures were not disclosed from the customer.)

Pharmaceuticals

Analysis of Mushroom extract on HP-C18

Analysis of Gentiopicroside on HP-C18

Analysis of Roxithromycin on HP-C18

Analysis of Validamycin A on HP-C18

Analysis of Amoxicillin on HP-C18

Analysis of Vitamins B6 and B12 on HP-C18

Analysis of Valsartan on HP-C18

Analysis of Cyclic Adenosine Monophospahte (cAMP) on HP-C18

Analysis of Florfenicol on HP-C18

Biochemistry

Separation of a Nucleotide mixture on HP-C18

Food Safety

Analysis of Melamine on HP-C18

Agriculture

Analysis of Imidacloprid on HP-C18

HP-C18

3 µm, 120 Å

3 µm, 200 Å

5 µm, 120 Å

5 µm, 200 Å

10 µm, 120 Å

10 µm, 200 Å

Sepax HP-C18

Part Number	Particle Size	Pore Size	ID×Length

103183-2001 ^[1]	3 µm	120 Å	2.0×10mm
103183-2001C ^[2]	3 µm	120 Å	2.0×10mm
103183-2103	3 µm	120 Å	2.1×30mm
103183-2105	3 µm	120 Å	2.1×50mm
103183-2110	3 µm	120 Å	2.1×100mm
103183-2115	3 µm	120 Å	2.1×150mm
103183-2125	3 µm	120 Å	2.1×250mm
103183-3003	3 µm	120 Å	3.0×30mm
103183-3005	3 µm	120 Å	3.0×50mm
103183-3010	3 µm	120 Å	3.0×100mm
103183-3015	3 µm	120 Å	3.0×150mm
103183-3025	3 µm	120 Å	3.0×250mm
103183-4001 ^[1]	3 µm	120 Å	4.0×10mm
103183-4001C ^[2]	3 µm	120 Å	4.0×10mm
103183-4603	3 µm	120 Å	4.6×30mm
103183-4605	3 µm	120 Å	4.6×50mm
103183-4610	3 µm	120 Å	4.6×100mm
103183-4615	3 µm	120 Å	4.6×150mm
103183-4625	3 µm	120 Å	4.6×250mm

[1]: Guard Cartridge only.
[2]: Guard Cartridge with holder (Part number ended with "C").

Precolumn Filters (Alternative choice for Guard Cartridge)
P/N#: 102000-P355 (2 µm PEEK frit), $65.00/ea
- for column with particle size 5 µm and 10 µm.
P/N#: 102000-P356 (0.5 µm PEEK frit), $65.00/ea
- for column with particle size 3 µm, or less than 3 µm.

PEEK Refill Frits (For Precolumn Filter)
P/N#: 102001-P355, 2 µm, 5 units/pk, $100/pk
P/N#: 102001-P356, 0.5 µm, 5 units/pk, $100/pk

Sepax HP-C18

Part Number	Particle Size	Pore Size	ID×Length

104183-2001 ^[1]	3 µm	200 Å	2.0×10mm
104183-2001C ^[2]	3 µm	200 Å	2.0×10mm
104183-2103	3 µm	200 Å	2.1×30mm
104183-2105	3 µm	200 Å	2.1×50mm
104183-2110	3 µm	200 Å	2.1×100mm
104183-2115	3 µm	200 Å	2.1×150mm
104183-2125	3 µm	200 Å	2.1×250mm
104183-3003	3 µm	200 Å	3.0×30mm
104183-3005	3 µm	200 Å	3.0×50mm
104183-3010	3 µm	200 Å	3.0×100mm
104183-3015	3 µm	200 Å	3.0×150mm
104183-3025	3 µm	200 Å	3.0×250mm
104183-4001 ^[1]	3 µm	200 Å	4.0×10mm
104183-4001C ^[2]	3 µm	200 Å	4.0×10mm
104183-4603	3 µm	200 Å	4.6×30mm
104183-4605	3 µm	200 Å	4.6×50mm
104183-4610	3 µm	200 Å	4.6×100mm
104183-4615	3 µm	200 Å	4.6×150mm
104183-4625	3 µm	200 Å	4.6×250mm

[1]: Guard Cartridge only.
[2]: Guard Cartridge with holder (Part number ended with "C").

PEEK Refill Frits (For Precolumn Filter)
P/N#: 102001-P355, 2 µm, 5 units/pk, $100/pk
P/N#: 102001-P356, 0.5 µm, 5 units/pk, $100/pk

Sepax HP-C18

Part Number	Particle Size	Pore Size	ID×Length

103185-2001 ^[1]	5 µm	120 Å	2.0×10mm
103185-2001C ^[2]	5 µm	120 Å	2.0×10mm
103185-2103	5 µm	120 Å	2.1×30mm
103185-2105	5 µm	120 Å	2.1×50mm
103185-2110	5 µm	120 Å	2.1×100mm
103185-2115	5 µm	120 Å	2.1×150mm
103185-2125	5 µm	120 Å	2.1×250mm
103185-3003	5 µm	120 Å	3.0×30mm
103185-3005	5 µm	120 Å	3.0×50mm
103185-3010	5 µm	120 Å	3.0×100mm
103185-3015	5 µm	120 Å	3.0×150mm
103185-3025	5 µm	120 Å	3.0×250mm
103185-4001 ^[1]	5 µm	120 Å	4.0×10mm
103185-4001C ^[2]	5 µm	120 Å	4.0×10mm
103185-4603	5 µm	120 Å	4.6×30mm
103185-4605	5 µm	120 Å	4.6×50mm
103185-4610	5 µm	120 Å	4.6×100mm
103185-4615	5 µm	120 Å	4.6×150mm
103185-4625	5 µm	120 Å	4.6×250mm
103185-7815	5 µm	120 Å	7.8×150mm
103185-7825	5 µm	120 Å	7.8×250mm
103185-10005	5 µm	120 Å	10.0×50mm
103185-10010	5 µm	120 Å	10.0×100mm
103185-10015	5 µm	120 Å	10.0×150mm
103185-10025	5 µm	120 Å	10.0×250mm
103185-21201 ^[1]	5 µm	120 Å	21.2×10mm
103185-21205	5 µm	120 Å	21.2×50mm
103185-21210	5 µm	120 Å	21.2×100mm
103185-21215	5 µm	120 Å	21.2×150mm
103185-21225	5 µm	120 Å	21.2×250mm
103185-30005	5 µm	120 Å	30.0×50mm
103185-30010	5 µm	120 Å	30.0×100mm
103185-30015	5 µm	120 Å	30.0×150mm
103185-30025	5 µm	120 Å	30.0×250mm

[1] Need guard cartridge holder
P/N#: 102000-21201
Price: $220.00/EA
(for 21.2×10mm guard cartridge)

Sepax HP-C18

Part Number	Particle Size	Pore Size	ID×Length

104185-2001 ^[1]	5 µm	200 Å	2.0×10mm
104185-2001C ^[2]	5 µm	200 Å	2.0×10mm
104185-2103	5 µm	200 Å	2.1×30mm
104185-2105	5 µm	200 Å	2.1×50mm
104185-2110	5 µm	200 Å	2.1×100mm
104185-2115	5 µm	200 Å	2.1×150mm
104185-2125	5 µm	200 Å	2.1×250mm
104185-3003	5 µm	200 Å	3.0×30mm
104185-3005	5 µm	200 Å	3.0×50mm
104185-3010	5 µm	200 Å	3.0×100mm
104185-3015	5 µm	200 Å	3.0×150mm
104185-3025	5 µm	200 Å	3.0×250mm
104185-4001 ^[1]	5 µm	200 Å	4.0×10mm
104185-4001C ^[2]	5 µm	200 Å	4.0×10mm
104185-4603	5 µm	200 Å	4.6×30mm
104185-4605	5 µm	200 Å	4.6×50mm
104185-4610	5 µm	200 Å	4.6×100mm
104185-4615	5 µm	200 Å	4.6×150mm
104185-4625	5 µm	200 Å	4.6×250mm
104185-7815	5 µm	200 Å	7.8×150mm
104185-7825	5 µm	200 Å	7.8×250mm
104185-10005	5 µm	200 Å	10.0×50mm
104185-10010	5 µm	200 Å	10.0×100mm
104185-10015	5 µm	200 Å	10.0×150mm
104185-10025	5 µm	200 Å	10.0×250mm
104185-21201 ^[1]	5 µm	200 Å	21.2×10mm
104185-21205	5 µm	200 Å	21.2×50mm
104185-21210	5 µm	200 Å	21.2×100mm
104185-21215	5 µm	200 Å	21.2×150mm
104185-21225	5 µm	200 Å	21.2×250mm
104185-30005	5 µm	200 Å	30.0×50mm
104185-30010	5 µm	200 Å	30.0×100mm
104185-30015	5 µm	200 Å	30.0×150mm
104185-30025	5 µm	200 Å	30.0×250mm

[1] Need guard cartridge holder
P/N#: 102000-21201
Price: $220.00/EA
(for 21.2×10mm guard cartridge)

Sepax HP-C18

Part Number	Particle Size	Pore Size	ID×Length

103189-7815	10 µm	120 Å	7.8×150mm
103189-7825	10 µm	120 Å	7.8×250mm
103189-10005	10 µm	120 Å	10.0×50mm
103189-10010	10 µm	120 Å	10.0×100mm
103189-10015	10 µm	120 Å	10.0×150mm
103189-10025	10 µm	120 Å	10.0×250mm
103189-21205	10 µm	120 Å	21.2×50mm
103189-21210	10 µm	120 Å	21.2×100mm
103189-21215	10 µm	120 Å	21.2×150mm
103189-21225	10 µm	120 Å	21.2×250mm
103189-30005	10 µm	120 Å	30.0×50mm
103189-30010	10 µm	120 Å	30.0×100mm
103189-30015	10 µm	120 Å	30.0×150mm
103189-30025	10 µm	120 Å	30.0×250mm

Sepax HP-C18

Part Number	Particle Size	Pore Size	ID×Length

104189-7815	10 µm	200 Å	7.8×150mm
104189-7825	10 µm	200 Å	7.8×250mm
104189-10005	10 µm	200 Å	10.0×50mm
104189-10010	10 µm	200 Å	10.0×100mm
104189-10015	10 µm	200 Å	10.0×150mm
104189-10025	10 µm	200 Å	10.0×250mm
104189-21205	10 µm	200 Å	21.2×50mm
104189-21210	10 µm	200 Å	21.2×100mm
104189-21215	10 µm	200 Å	21.2×150mm
104189-21225	10 µm	200 Å	21.2×250mm
104189-30005	10 µm	200 Å	30.0×50mm
104189-30010	10 µm	200 Å	30.0×100mm
104189-30015	10 µm	200 Å	30.0×150mm
104189-30025	10 µm	200 Å	30.0×250mm

Catalog

User Manual