Sepax HP-C18 HPLC Columns

HP-C18 uses full coverage bonded silica packing, which provides exceptionally high stability. Compatible with 100% aqueous mobile phase suitable for separations of acidic, neutral and basic organic compounds, as well as pharmaceuticals and peptides.

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• User Manual

ODS monolayer formed by special bonding chemistry does not collapse in pure aqueous solution.

Silica:	Spherical, high purity (<10 ppm metals)
Pore size:	120 Å
Particle size:	3, 4, 5, 7, and 10 µm
Pore volume:	1.0 ml/g
Surface area:	300 m²/g
Phase structure:	Monomeric and fully endcapped
% Carbon:	17%
Pore size:	200 Å
Particle size:	3 and 5 µm
Pore volume:	1.0 ml/g
Surface area:	200 m²/g
Phase structure:	Monomeric and fully endcapped
% Carbon:	10%

• Highly controlled chemistry of monolayer formation and end-capping
• Extremely high column-to-column reproducibility
• High selectivity and efficiency for separations
• Compatible with 100% aqueous mobile phase
• Suitable for separations of acidic, neutral and basic organic compounds, as well as pharmaceuticals, peptides, and others

Sepax HP-C18 uses full coverage bonded silica packing, which provides the exceptionally high stability. Figure 1 shows the highly reproducible retention time for three standard compounds: aniline, anisole and toluene after 13,000 column volume runs in a mobile phase of 85% methanol and 15% water. Such high stability allows Sepax HP-C18 extremely suitable for validation of various analytes.

Figure shown below is a real application of Sepax HP-C18, showing fast separation of 8 pharmaceutical molecules by a 3 µm, 4.6×50mm short column. After 1800 column volume of "0.1% TFA and CH₃CN mobile phase" was pumped, the column still shows almost identical performance!

Sepax HP-C18 has better selectivity and resolution for organometallic complex isomers compared to other competitors' C18 phases.

Organic Acids
Purine Alkaloids
Catecholamines

Sepax HP-C18 Part Number Particle Size Pore Size ID×Length Price 103183-2001 * 3 µm 120Å 2.0×10mm $165.00 103183-2103 3 µm 120Å 2.1×30mm $350.00 103183-2105 3 µm 120Å 2.1×50mm $350.00 103183-2110 3 µm 120Å 2.1×100mm $415.00 103183-2115 3 µm 120Å 2.1×150mm $460.00 103183-2125 3 µm 120Å 2.1×250mm $515.00 103183-3003 3 µm 120Å 3.0×30mm $350.00 103183-3005 3 µm 120Å 3.0×50mm $350.00 103183-3010 3 µm 120Å 3.0×100mm $415.00 103183-3015 3 µm 120Å 3.0×150mm $460.00 103183-3025 3 µm 120Å 3.0×250mm $515.00 103183-4001 * 3 µm 120Å 4.0×10mm $165.00 103183-4603 3 µm 120Å 4.6×30mm $350.00 103183-4605 3 µm 120Å 4.6×50mm $350.00 103183-4610 3 µm 120Å 4.6×100mm $415.00 103183-4615 3 µm 120Å 4.6×150mm $460.00 103183-4625 3 µm 120Å 4.6×250mm $515.00 104183-2001 * 3 µm 200Å 2.0×10mm $165.00 104183-2103 3 µm 200Å 2.1×30mm $350.00 104183-2105 3 µm 200Å 2.1×50mm $350.00 104183-2110 3 µm 200Å 2.1×100mm $415.00 104183-2115 3 µm 200Å 2.1×150mm $460.00 104183-2125 3 µm 200Å 2.1×250mm $515.00 104183-3003 3 µm 200Å 3.0×30mm $350.00 104183-3005 3 µm 200Å 3.0×50mm $350.00 104183-3010 3 µm 200Å 3.0×100mm $415.00 104183-3015 3 µm 200Å 3.0×150mm $460.00 104183-3025 3 µm 200Å 3.0×250mm $515.00 104183-4001 * 3 µm 200Å 4.0×10mm $165.00 104183-4603 3 µm 200Å 4.6×30mm $350.00 104183-4605 3 µm 200Å 4.6×50mm $350.00 104183-4610 3 µm 200Å 4.6×100mm $415.00 104183-4615 3 µm 200Å 4.6×150mm $460.00 104183-4625 3 µm 200Å 4.6×250mm $515.00 * Guard cartridge holder needed. Guard cartridge holder P/N#: 102000-2001, $42.50/EA, for 2.0×10mm guard cartridge P/N#: 102000-4001, $42.50/EA, for 4.0×10mm guard cartridge Sepax HP-C18 Part Number Particle Size Pore Size ID×Length Price 103184-2001 * 4 µm 120Å 2.0×10mm $165.00 103184-2103 4 µm 120Å 2.1×30mm $350.00 103184-2105 4 µm 120Å 2.1×50mm $350.00 103184-2110 4 µm 120Å 2.1×100mm $415.00 103184-2115 4 µm 120Å 2.1×150mm $460.00 103184-2125 4 µm 120Å 2.1×250mm $515.00 103184-3003 4 µm 120Å 3.0×30mm $350.00 103184-3005 4 µm 120Å 3.0×50mm $350.00 103184-3010 4 µm 120Å 3.0×100mm $415.00 103184-3015 4 µm 120Å 3.0×150mm $460.00 103184-3025 4 µm 120Å 3.0×250mm $515.00 103184-4001 * 4 µm 120Å 4.0×10mm $165.00 103184-4603 4 µm 120Å 4.6×30mm $350.00 103184-4605 4 µm 120Å 4.6×50mm $350.00 103184-4610 4 µm 120Å 4.6×100mm $415.00 103184-4615 4 µm 120Å 4.6×150mm $460.00 103184-4625 4 µm 120Å 4.6×250mm $515.00 * Guard cartridge holder needed. Guard cartridge holder P/N#: 102000-2001, $42.50/EA, for 2.0×10mm guard cartridge P/N#: 102000-4001, $42.50/EA, for 4.0×10mm guard cartridge Sepax HP-C18 Part Number Particle Size Pore Size ID×Length Price 103185-2001 * 5 µm 120Å 2.0×10mm $165.00 103185-2103 5 µm 120Å 2.1×30mm $330.00 103185-2105 5 µm 120Å 2.1×50mm $330.00 103185-2110 5 µm 120Å 2.1×100mm $390.00 103185-2115 5 µm 120Å 2.1×150mm $445.00 103185-2125 5 µm 120Å 2.1×250mm $500.00 103185-3003 5 µm 120Å 3.0×30mm $330.00 103185-3005 5 µm 120Å 3.0×50mm $330.00 103185-3010 5 µm 120Å 3.0×100mm $390.00 103185-3015 5 µm 120Å 3.0×150mm $445.00 103185-3025 5 µm 120Å 3.0×250mm $500.00 103185-4001 * 5 µm 120Å 4.0×10mm $165.00 103185-4603 5 µm 120Å 4.6×30mm $330.00 103185-4605 5 µm 120Å 4.6×50mm $330.00 103185-4610 5 µm 120Å 4.6×100mm $390.00 103185-4615 5 µm 120Å 4.6×150mm $445.00 103185-4625 5 µm 120Å 4.6×250mm $500.00 104185-2001 * 5 µm 200Å 2.0×10mm $165.00 104185-2103 5 µm 200Å 2.1×30mm $330.00 104185-2105 5 µm 200Å 2.1×50mm $330.00 104185-2110 5 µm 200Å 2.1×100mm $390.00 104185-2115 5 µm 200Å 2.1×150mm $445.00 104185-2125 5 µm 200Å 2.1×250mm $500.00 104185-3003 5 µm 200Å 3.0×30mm $330.00 104185-3005 5 µm 200Å 3.0×50mm $330.00 104185-3010 5 µm 200Å 3.0×100mm $390.00 104185-3015 5 µm 200Å 3.0×150mm $445.00 104185-3025 5 µm 200Å 3.0×250mm $500.00 104185-4001 * 5 µm 200Å 4.0×10mm $165.00 104185-4603 5 µm 200Å 4.6×30mm $330.00 104185-4605 5 µm 200Å 4.6×50mm $330.00 104185-4610 5 µm 200Å 4.6×100mm $390.00 104185-4615 5 µm 200Å 4.6×150mm $445.00 104185-4625 5 µm 200Å 4.6×250mm $500.00 * Guard cartridge holder needed. Guard cartridge holder P/N#: 102000-2001, $42.50/EA, for 2.0×10mm guard cartridge P/N#: 102000-4001, $42.50/EA, for 4.0×10mm guard cartridge Sepax HP-C18 Part Number Particle Size Pore Size ID×Length Price 103185-7815 5 µm 120Å 7.8×150mm $910.00 103185-7825 5 µm 120Å 7.8×250mm $1,040.00 103185-10005 5 µm 120Å 10.0×50mm $525.00 103185-10010 5 µm 120Å 10.0×100mm $910.00 103185-10015 5 µm 120Å 10.0×150mm $1,030.00 103185-10025 5 µm 120Å 10.0×250mm $1,265.00 103185-21201 * 5 µm 120Å 21.2×10mm $305.00 103185-21205 5 µm 120Å 21.2×50mm $1,515.00 103185-21210 5 µm 120Å 21.2×100mm $2,040.00 103185-21215 5 µm 120Å 21.2×150mm $2,305.00 103185-21225 5 µm 120Å 21.2×250mm $2,695.00 103185-30005 5 µm 120Å 30.0×50mm $2,530.00 103185-30010 5 µm 120Å 30.0×100mm $3,085.00 103185-30015 5 µm 120Å 30.0×150mm $3,910.00 103185-30025 5 µm 120Å 30.0×250mm $5,330.00 104185-7815 5 µm 200Å 7.8×150mm $910.00 104185-7825 5 µm 200Å 7.8×250mm $1,040.00 104185-10005 5 µm 200Å 10.0×50mm $525.00 104185-10010 5 µm 200Å 10.0×100mm $910.00 104185-10015 5 µm 200Å 10.0×150mm $1,030.00 104185-10025 5 µm 200Å 10.0×250mm $1,265.00 104185-21201 * 5 µm 200Å 21.2×10mm $305.00 104185-21205 5 µm 200Å 21.2×50mm $1,515.00 104185-21210 5 µm 200Å 21.2×100mm $2,040.00 104185-21215 5 µm 200Å 21.2×150mm $2,305.00 104185-21225 5 µm 200Å 21.2×250mm $2,695.00 104185-30005 5 µm 200Å 30.0×50mm $2,530.00 104185-30010 5 µm 200Å 30.0×100mm $3,085.00 104185-30015 5 µm 200Å 30.0×150mm $3,910.00 104185-30025 5 µm 200Å 30.0×250mm $5,330.00 * Guard cartridge holder needed. Guard cartridge holder P/N#: 102000-21201 Price: $175.00/EA (for 21.2×10mm guard cartridge) Sepax HP-C18 Part Number Particle Size Pore Size ID×Length Price 103187-7815 7 µm 120Å 7.8×150mm $765.00 103187-7825 7 µm 120Å 7.8×250mm $940.00 103187-10005 7 µm 120Å 10.0×50mm $435.00 103187-10010 7 µm 120Å 10.0×100mm $765.00 103187-10015 7 µm 120Å 10.0×150mm $985.00 103187-10025 7 µm 120Å 10.0×250mm $1,265.00 103187-21201 * 7 µm 120Å 21.2×10mm $305.00 103187-21205 7 µm 120Å 21.2×50mm $1,185.00 103187-21210 7 µm 120Å 21.2×100mm $1,735.00 103187-21215 7 µm 120Å 21.2×150mm $2,065.00 103187-21225 7 µm 120Å 21.2×250mm $2,305.00 103187-30025 7 µm 120Å 30.0×250mm Inquire * Guard cartridge holder needed. Guard cartridge holder P/N#: 102000-21201 Price: $175.00/EA (for 21.2×10mm guard cartridge) Sepax HP-C18 Part Number Particle Size Pore Size ID×Length Price 103189-7815 10 µm 120Å 7.8×150mm $635.00 103189-7825 10 µm 120Å 7.8×250mm $765.00 103189-10005 10 µm 120Å 10.0×50mm $415.00 103189-10010 10 µm 120Å 10.0×100mm $690.00 103189-10015 10 µm 120Å 10.0×150mm $875.00 103189-10025 10 µm 120Å 10.0×250mm $1,020.00 103189-21205 10 µm 120Å 21.2×50mm $1,045.00 103189-21210 10 µm 120Å 21.2×100mm $1,625.00 103189-21215 10 µm 120Å 21.2×150mm $1,820.00 103189-21225 10 µm 120Å 21.2×250mm $2,145.00 103189-30005 10 µm 120Å 30.0×50mm $2,040.00 103189-30010 10 µm 120Å 30.0×100mm $2,530.00 103189-30015 10 µm 120Å 30.0×150mm $3,495.00 103189-30025 10 µm 120Å 30.0×250mm $4,265.00 104189-7815 10 µm 200Å 7.8×150mm $635.00 104189-7825 10 µm 200Å 7.8×250mm $765.00 104189-10005 10 µm 200Å 10.0×50mm $415.00 104189-10010 10 µm 200Å 10.0×100mm $690.00 104189-10015 10 µm 200Å 10.0×150mm $875.00 104189-10025 10 µm 200Å 10.0×250mm $1,020.00 104189-21205 10 µm 200Å 21.2×50mm $1,045.00 104189-21210 10 µm 200Å 21.2×100mm $1,625.00 104189-21215 10 µm 200Å 21.2×150mm $1,820.00 104189-21225 10 µm 200Å 21.2×250mm $2,145.00 104189-30005 10 µm 200Å 30.0×50mm $2,040.00 104189-30010 10 µm 200Å 30.0×100mm $2,530.00 104189-30015 10 µm 200Å 30.0×150mm $3,495.00 104189-30025 10 µm 200Å 30.0×250mm $4,265.00

Analytical HP-C18, 3µm HP-C18, 4µm HP-C18, 5µm Prep/Semi-prep HP-C18, 5µm HP-C18, 7µm HP-C18, 10µm