Utilizing highest purity and enhanced mechanical stability silica and pure bonding reagents, BR-C18 bonded phases have been innovatively and specially designed to ensure maximum surface coverage and full end-capping, which leads to carbon content as high as 19.0%. The bonding chemistry is completely controlled that results in very reliable column-to-column reproducibility. The maximum surface coverage allows BR-C18 to have exceptional stability, resulting in high pH stability in the range of 1.5 to 10.5.

Silica:	Spherical, high purity (<10 ppm metals)
Pore size:	120Å
Particle size:	3, 5, and 10 µm
Pore volume:	1.0 ml/g
Surface area:	350 m²/g
Phase structure:	Monomeric and fully endcapped
% Carbon:	19.0%

• Highly controlled chemistry of monolayer formation and end-capping
• Extremely high column-to-column reproducibility
• High selectivity and efficiency for separations
• pH range 1.5-10.5
• Suitable for separations of acidic, neutral and basic compounds, peptides, and proteins

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Column Stability at Alkali Conditions

BR-C18 uses full coverage bonded silica packing, which allows high stability at high pH. Figure below shows reproducible retention time for a test compound: toluene after 18,000 column volume runs in a mobile phase of 55% acetonitrile and 45% water at pH 10. Such high stability allows BR-C18 well suited for validation of various analytes at alkali conditions. The proprietary bonding chemistry for BR-C18 allows achieving high selectivity and high efficiency separation.

A BR-C18 column was operated at pH 10.

Conditions Column: BR-C18, 3 µm, 4.6×50 mm Mobile phase: 10 mM ammonium bicarbonate buffer in 55%ACN/45%H2O Temperature: Ambient Flow rate: 0.5 mL/min Detector: UV 254 nm Sample: Toluene

BR-C18      1.8 µm         2.2 µm         3 µm         5 µm         10 µm

Sepax BR-C18 Sign In to see pricing Part Number Particle Size Pore Size ID×Length 102181-2103 1.8 µm 120 Å 2.1×30mm 102181-2105 1.8 µm 120 Å 2.1×50mm 102181-2110 1.8 µm 120 Å 2.1×100mm 102181-2115 1.8 µm 120 Å 2.1×150mm 102181-3003 1.8 µm 120 Å 3.0×30mm 102181-3005 1.8 µm 120 Å 3.0×50mm 102181-3010 1.8 µm 120 Å 3.0×100mm 102181-3015 1.8 µm 120 Å 3.0×150mm 102181-4603 1.8 µm 120 Å 4.6×30mm 102181-4605 1.8 µm 120 Å 4.6×50mm 102181-4610 1.8 µm 120 Å 4.6×100mm 102181-4615 1.8 µm 120 Å 4.6×150mm Sepax BR-C18 Sign In to see pricing Part Number Particle Size Pore Size ID×Length 102182-2103 2.2 µm 120 Å 2.1×30mm 102182-2105 2.2 µm 120 Å 2.1×50mm 102182-2110 2.2 µm 120 Å 2.1×100mm 102182-2115 2.2 µm 120 Å 2.1×150mm 102182-3003 2.2 µm 120 Å 3.0×30mm 102182-3005 2.2 µm 120 Å 3.0×50mm 102182-3010 2.2 µm 120 Å 3.0×100mm 102182-3015 2.2 µm 120 Å 3.0×150mm 102182-4603 2.2 µm 120 Å 4.6×30mm 102182-4605 2.2 µm 120 Å 4.6×50mm 102182-4610 2.2 µm 120 Å 4.6×100mm 102182-4615 2.2 µm 120 Å 4.6×150mm Sepax BR-C18 Sign In to see pricing Part Number Particle Size Pore Size ID×Length 102183-2001 [1] 3 µm 120 Å 2.0×10mm 102183-2001C [2] 3 µm 120 Å 2.0×10mm 102183-2103 3 µm 120 Å 2.1×30mm 102183-2105 3 µm 120 Å 2.1×50mm 102183-2110 3 µm 120 Å 2.1×100mm 102183-2115 3 µm 120 Å 2.1×150mm 102183-2125 3 µm 120 Å 2.1×250mm 102183-3003 3 µm 120 Å 3.0×30mm 102183-3005 3 µm 120 Å 3.0×50mm 102183-3010 3 µm 120 Å 3.0×100mm 102183-3015 3 µm 120 Å 3.0×150mm 102183-3025 3 µm 120 Å 3.0×250mm 102183-4001 [1] 3 µm 120 Å 4.0×10mm 102183-4001C [2] 3 µm 120 Å 4.0×10mm 102183-4603 3 µm 120 Å 4.6×30mm 102183-4605 3 µm 120 Å 4.6×50mm 102183-4610 3 µm 120 Å 4.6×100mm 102183-4615 3 µm 120 Å 4.6×150mm 102183-4625 3 µm 120 Å 4.6×250mm [1]: Guard Cartridge only. [2]: Guard Cartridge with holder (Part number ended with "C"). Precolumn Filters (Alternative choice for Guard Cartridge) P/N#: 102000-P355 (2 µm PEEK frit), $65.00/ea     - for column with particle size 5 µm and 10 µm. P/N#: 102000-P356 (0.5 µm PEEK frit), $65.00/ea     - for column with particle size 3 µm, or less than 3 µm. PEEK Refill Frits (For Precolumn Filter) P/N#: 102001-P355, 2 µm, 5 units/pk, $100/pk P/N#: 102001-P356, 0.5 µm, 5 units/pk, $100/pk Sepax BR-C18 Sign In to see pricing Part Number Particle Size Pore Size ID×Length 102185-2001 [1] 5 µm 120 Å 2.0×10mm 102185-2001C [2] 5 µm 120 Å 2.0×10mm 102185-2103 5 µm 120 Å 2.1×30mm 102185-2105 5 µm 120 Å 2.1×50mm 102185-2110 5 µm 120 Å 2.1×100mm 102185-2115 5 µm 120 Å 2.1×150mm 102185-2125 5 µm 120 Å 2.1×250mm 102185-3003 5 µm 120 Å 3.0×30mm 102185-3005 5 µm 120 Å 3.0×50mm 102185-3010 5 µm 120 Å 3.0×100mm 102185-3015 5 µm 120 Å 3.0×150mm 102185-3025 5 µm 120 Å 3.0×250mm 102185-4001 [1] 5 µm 120 Å 4.0×10mm 102185-4001C [2] 5 µm 120 Å 4.0×10mm 102185-4603 5 µm 120 Å 4.6×30mm 102185-4605 5 µm 120 Å 4.6×50mm 102185-4610 5 µm 120 Å 4.6×100mm 102185-4615 5 µm 120 Å 4.6×150mm 102185-4625 5 µm 120 Å 4.6×250mm 102185-7815 5 µm 120 Å 7.8×150mm 102185-7825 5 µm 120 Å 7.8×250mm 102185-10005 5 µm 120 Å 10.0×50mm 102185-10010 5 µm 120 Å 10.0×100mm 102185-10015 5 µm 120 Å 10.0×150mm 102185-10025 5 µm 120 Å 10.0×250mm 102185-21201 [1] 5 µm 120 Å 21.2×10mm 102185-21205 5 µm 120 Å 21.2×50mm 102185-21210 5 µm 120 Å 21.2×100mm 102185-21215 5 µm 120 Å 21.2×150mm 102185-21225 5 µm 120 Å 21.2×250mm 102185-30005 5 µm 120 Å 30.0×50mm 102185-30010 5 µm 120 Å 30.0×100mm 102185-30015 5 µm 120 Å 30.0×150mm 102185-30025 5 µm 120 Å 30.0×250mm [1] Need guard cartridge holder P/N#: 102000-21201 Price: $220.00/EA (for 21.2×10mm guard cartridge) Sepax BR-C18 Sign In to see pricing Part Number Particle Size Pore Size ID×Length 102189-7815 10 µm 120 Å 7.8×150mm 102189-7825 10 µm 120 Å 7.8×250mm 102189-10005 10 µm 120 Å 10.0×50mm 102189-10010 10 µm 120 Å 10.0×100mm 102189-10015 10 µm 120 Å 10.0×150mm 102189-10025 10 µm 120 Å 10.0×250mm 102189-21205 10 µm 120 Å 21.2×50mm 102189-21210 10 µm 120 Å 21.2×100mm 102189-21215 10 µm 120 Å 21.2×150mm 102189-21225 10 µm 120 Å 21.2×250mm 102189-30005 10 µm 120 Å 30.0×50mm 102189-30010 10 µm 120 Å 30.0×100mm 102189-30015 10 µm 120 Å 30.0×150mm 102189-30025 10 µm 120 Å 30.0×250mm

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