Utilizing highest purity and enhanced mechanical stability silica and pure bonding reagents, BR-C18 bonded phases have been innovatively and specially designed to ensure maximum surface coverage and full end-capping, which leads to carbon content as high as 19.0%. The bonding chemistry is completely controlled that results in very reliable column-to-column reproducibility. The maximum surface coverage allows BR-C18 to have exceptional stability, resulting in high pH stability in the range of 1.5 to 10.5.
Silica:
Spherical, high purity (<10 ppm metals)
Pore size:
120Å
Particle size:
3, 5, and 10 µm
Pore volume:
1.0 ml/g
Surface area:
350 m²/g
Phase structure:
Monomeric and fully endcapped
% Carbon:
19.0%
Highly controlled chemistry of monolayer formation and end-capping
Extremely high column-to-column reproducibility
High selectivity and efficiency for separations
pH range 1.5-10.5
Suitable for separations of acidic, neutral and basic compounds, peptides, and proteins
Column Stability at Alkali Conditions
BR-C18 uses full coverage bonded silica packing, which allows high stability at high pH. Figure below shows reproducible retention time for a test compound: toluene after 18,000 column volume runs in a mobile phase of 55% acetonitrile and 45% water at pH 10. Such high stability allows BR-C18 well suited for validation of various analytes at alkali conditions. The proprietary bonding chemistry for BR-C18 allows achieving high selectivity and high efficiency separation.
A BR-C18 column was operated at pH 10.
Conditions
Column:
BR-C18, 3 µm, 4.6×50 mm
Mobile phase:
10 mM ammonium bicarbonate buffer in 55%ACN/45%H2O
[1] Guard column [2] One-piece PEEK coupler, recommended to connect columns in series. [3] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [4] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [5] For 21.2x10mm guard cartridge
[1] Guard column [2] Guard Cartridge only. [3] Guard Cartridge with holder. [4] One-piece PEEK coupler, recommended to connect columns in series. [5] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [6] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [7] For 21.2x10mm guard cartridge
[1] Guard column [2] Guard Cartridge only. [3] Guard Cartridge with holder. [4] One-piece PEEK coupler, recommended to connect columns in series. [5] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [6] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [7] For 21.2x10mm guard cartridge
[1] Guard column [2] One-piece PEEK coupler, recommended to connect columns in series. [3] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [4] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [5] For 21.2x10mm guard cartridge
