Sepax Carbomix NP Columns

Carbomix phases are composed of spherical, low cross-linking polystyrene/ divinylbenzene (PS/DVB) particles with their surfaces modified with sulfonic acid (-SO₃H), followed by chelating of calcium ions (Ca⁺²) or other metal ions. The PS/DVB support has cross-linking degree of 5%, 8% and 10%. The standard Carbomix packings for analytical applications are 5 µm uniform particles. Carbomix resins of 10 µm and above particle size are manufactured for preparative separation applications. Carbomix resins have been specifically designed for high resolution separation of carbohydrates, organic acids, peptides, and nucleic acids.

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Carbomix Ca-NP phases

Support:	spherical, PS/DVB particles
Cross-linking:	5%, 8% and 10%
Particle size:	5 and 10 µm
Phase structure:	hydrophilic, ion-exchange
Chemical composition:	PS/DVB-(SO3-)2Ca2+

Carbomix Ca-NP resins have been specifically designed for high resolution separation of carbohydrates, organic acids, peptides, and nucleic acids. These novel packing materials are based on low crosslinked polystyrene / divinylbenzene (PS/DVB) particles with the surface modified with sulfonic acid (-SO₃H), followed by chelating of calcium ions (Ca⁺²). Their narrow particle size distribution offers high efficiency and high resolution separations. The low cross-linking generates swelling for the resin in the mobile phase, resulting in reasonable surface area and capacity. Figure 1 is a typical test chromatogram for the separation of carbohydrates by a Carbomix Ca-NP5 column.

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Carbomix H-NP Series

Support:	spherical, PS/DVB particles
Cross-linking:	5%, 8% and 10%
Particle size:	5 and 10 µm
Phase structure:	hydrophilic, ion-exchange
Chemical composition:	PS/DVB-SO3H

Carbomix H-NP resins have been specifically designed for high resolution separation of carbohydrates, organic acids, peptides, and nucleic acids. These novel packing materials are based on low crosslinked polystyrene / divinylbenzene (PS/DVB) particles with the surface modified with sulfonic acid (-SO₃H). Their narrow particle size distribution offers high efficiency and high resolution separations. The low cross-linking generates swelling for the resin in the mobile phase, resulting in reasonable surface area and capacity.

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Technical Specifications

Phase	Carbomix Ca-NP	Carbomix H-NP
Support	Non-porous PS/DVB	Non-porous PS/DVB
Particle size	5, 10 µm	5, 10 µm
Cross-linking	5%, 8% and 10%	5%, 8% and 10%
Stationary phase	-(SO3)2-Ca2+	-SO3H
pH stability	5 - 9	1 - 3
Typical backpressure (psi)	~ 700 (0.6 mL/min for a 7.8×300 mm column)	~ 700 (0.6 mL/min for a 7.8×300 mm column)
Maximum backpressure (psi)	~ 2,500	~ 2,500
Typical mobile phase	H2O	5 mM H2SO4, H2O
Typical flow rate(mL/min)	0.4-0.8 for a 7.8×300mm column 0.1-0.35 for a 4.6×300mm column	0.4-0.8 for a 7.8×300mm column 0.1-0.35 for a 4.6×300mm column
Maximum temperature (°C)	~ 85	~ 65

• Uniform particle size for high resolution and efficiency separation
• Compatibility with most aqueous mobile phases, including pure water as the eluent
• Wide range of operating temperature (0-85 °C)
• Low backpressure for the separation
• pH range (5-9) for Carbomix Ca-NP and (1-3) for Carbomix H-NP columns

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Separation Mechanism

The separation mechanism for the Carbomix Ca-NP phases includes ion-exchange and hydrophilic interactions with the analytes, such as carbohydrates and organic acids. The separation mechanism could also be due to size exclusion, ion exclusion, and ligand exchange. These multiple modes of interaction enable a unique capability to separate a variety of water soluble compounds. Resin cross-linking degree is an important parameter in the separation. Styrene divinylbenzene resin is a relatively rigid gel-type media. The lower the cross-linking, the more open the pore structure, and the more permeable it is to higher molecular weight substances. A 5% crosslinked Carbomix resin can resolve higher oligosaccharides compared to 10% cross-linked resin. For smaller molecular weight compounds an 8% crosslinked resin is used.

High Stability

The Carbomix resins are stable in pure water and other aqueous buffers at elevated temperature. The reproducibility from run to run is very consistent. Figure below shows the retention time variation for 7 runs is only 0.1% for a Carbomix Ca-NP5 column.

High Lot to Lot Reproducibility

With well controlled polymer resin production and the surface chemistry, the manufacturing of the Carbomix ion-exchangers is highly reproducible. The typical variation of the retention time is less than 6% from batch to batch.

Column Configuration

Carbomix resins can be packed into wide range of column dimensions with ID from 75 mm to 21.2 mm and the length from 5 cm to 30 cm. A custom-made column is also available. Column length and diameter affect resolution and analysis time. The selection of the column is to use only as much resin as necessary to achieve the desired separation. If the compound is strongly retained by the resin and analysis time is long on a 300 × 7.8 mm column, a shorter column, such as 150 mm long can significantly decrease the analysis time.

The Carbomix resins and columns offer many advantages for the analysis of carbohydrates, alcohols, and organic acids in food, beverage, biochemical, biomedical, and biotechnology applications.

Organic acid and alcohol analysis include sugars with organic acids, alcohol, glycol, and fermentation products. Carbohydrate analysis includes samples of beet sugars, molasses, corn syrup, pentose sugars, cellulose hydrolysates, oligosaccharides, glucose, galactose, sucrose, and fructose.

Typical industrial applications areas:

• Food and Beverage
• Fruits and Vegetables
• Wine and Beer
• Clinical Applications
• Oligosaccharides Analysis
• Cellulose and Wood
• Plant Biochemistry
• Fermentation Monitoring
• Metabolite Analysis
• Bacteria and Yeast Analysis
• Glycoproteins and Glycoconjugates
• Nucleic Acids

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Separation of carbohydrates in beer

Separation of carbohydrates in food
Separation of glucose and its oligomers
Separation of monosaccharide
Separation of glucidex mixture

Sepax Carbomix Ca-NP5, Non-Porous Part Number Particle Size Cross-Linking ID×Length Price 250505-4625 5 µm 5% 4.6×250mm $575.00 250508-4625 5 µm 8% 4.6×250mm $575.00 250510-4625 5 µm 10% 4.6×250mm $575.00 250505-4630 5 µm 5% 4.6×300mm $635.00 250508-4630 5 µm 8% 4.6×300mm $635.00 250510-4630 5 µm 10% 4.6×300mm $635.00 250505-7830 5 µm 5% 7.8×300mm $835.00 250508-7830 5 µm 8% 7.8×300mm $835.00 250510-7830 5 µm 10% 7.8×300mm $835.00 Sepax Carbomix Ca-NP10, Non-Porous Part Number Particle Size Cross-Linking ID×Length Price 251005-4625 10 µm 5% 4.6×250mm $485.00 251008-4625 10 µm 8% 4.6×250mm $485.00 251010-4625 10 µm 10% 4.6×250mm $485.00 251005-4630 10 µm 5% 4.6×300mm $540.00 251008-4630 10 µm 8% 4.6×300mm $540.00 251010-4630 10 µm 10% 4.6×300mm $540.00 251005-7830 10 µm 5% 7.8×300mm $710.00 251008-7830 10 µm 8% 7.8×300mm $710.00 251010-7830 10 µm 10% 7.8×300mm $710.00 Sepax Carbomix H-NP5, Non-Porous Part Number Particle Size Cross-Linking ID×Length Price 260505-4625 5 µm 5% 4.6×250mm $575.00 260508-4625 5 µm 8% 4.6×250mm $575.00 260510-4625 5 µm 10% 4.6×250mm $575.00 260505-4630 5 µm 5% 4.6×300mm $635.00 260508-4630 5 µm 8% 4.6×300mm $635.00 260510-4630 5 µm 10% 4.6×300mm $635.00 260505-7830 5 µm 5% 7.8×300mm $835.00 260508-7830 5 µm 8% 7.8×300mm $835.00 260510-7830 5 µm 10% 7.8×300mm $835.00 Sepax Carbomix H-NP10, Non-Porous Part Number Particle Size Cross-Linking ID×Length Price 261005-4625 10 µm 5% 4.6×250mm $485.00 261008-4625 10 µm 8% 4.6×250mm $485.00 261010-4625 10 µm 10% 4.6×250mm $485.00 261005-4630 10 µm 5% 4.6×300mm $540.00 261008-4630 10 µm 8% 4.6×300mm $540.00 261010-4630 10 µm 10% 4.6×300mm $540.00 261005-7830 10 µm 5% 7.8×300mm $710.00 261008-7830 10 µm 8% 7.8×300mm $710.00 261010-7830 10 µm 10% 7.8×300mm $710.00 Sepax Carbomix Ca-NP5, Non-Porous Part Number Particle Size Cross-Linking ID×Length Price 250505-10030 5 µm 5% 10.0×300mm $1,285.00 250508-10030 5 µm 8% 10.0×300mm $1,285.00 250510-10030 5 µm 10% 10.0×300mm $1,285.00 250505-21225 5 µm 5% 21.2×250mm Inquire 250508-21225 5 µm 8% 21.2×250mm Inquire 250510-21225 5 µm 10% 21.2×250mm Inquire Sepax Carbomix Ca-NP10, Non-Porous Part Number Particle Size Cross-Linking ID×Length Price 251005-10030 10 µm 5% 10.0×300mm $1,090.00 251008-10030 10 µm 8% 10.0×300mm $1,090.00 251010-10030 10 µm 10% 10.0×300mm $1,090.00 251005-21225 10 µm 5% 21.2×250mm Inquire 251008-21225 10 µm 8% 21.2×250mm Inquire 251010-21225 10 µm 10% 21.2×250mm Inquire Sepax Carbomix H-NP5, Non-Porous Part Number Particle Size Cross-Linking ID×Length Price 260505-10030 5 µm 5% 10.0×300mm $1,285.00 260508-10030 5 µm 8% 10.0×300mm $1,285.00 260510-10030 5 µm 10% 10.0×300mm $1,285.00 260505-21225 5 µm 5% 21.2×250mm Inquire 260508-21225 5 µm 8% 21.2×250mm Inquire 260510-21225 5 µm 10% 21.2×250mm Inquire Sepax Carbomix H-NP10, Non-Porous Part Number Particle Size Cross-Linking ID×Length Price 261005-10030 10 µm 5% 10.0×300mm $1,090.00 261008-10030 10 µm 8% 10.0×300mm $1,090.00 261010-10030 10 µm 10% 10.0×300mm $1,090.00 261005-21225 10 µm 5% 21.2×250mm Inquire 261008-21225 10 µm 8% 21.2×250mm Inquire 261010-21225 10 µm 10% 21.2×250mm Inquire

Analytical Carbomix Ca, 5µm Carbomix Ca, 10µm Carbomix H, 5µm Carbomix H, 10µm Prep/Semi-prep Carbomix Ca, 5µm Carbomix Ca, 10µm Carbomix H, 5µm Carbomix H, 10µm