GP-C18 uses full coverage bonded silica packing, which provides exceptionally high stability. The unique mono-functional bonding chemistry of GP-C18 avoids the formation of multiple C18 layers. Such uniform stationary phase allows the separation to achieve high selectivity and high efficiency.

Silica:	Spherical, high purity (<10 ppm metals)
Pore size:	120Å
Particle size:	1.8, 2.2, 3, 4, 5, 7, and 10 µm
Pore volume:	1.0 ml/g
Surface area:	300 m²/g
Phase structure:	Monomeric and fully endcapped
% Carbon:	17%

• Highly controlled chemistry of monolayer formation and end-capping
• Extremely high column-to-column reproducibility
• High selectivity and efficiency for separations
• Suitable for separations of acidic, neutral and basic organic compounds, as well as pharmaceuticals, peptides, and others
• Recommended for separations in organic or mixed organic/aqueous mobile phases.

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Stability

SepaxGP-C18 uses full coverage bonded silica packing, which provides exceptionally high stability. Figure below shows the highly reproducible retention time for three standard compounds: aniline, anisole and toluene after 13,000 column volume runs in a mobile phase of 85% methanol and 15% water. Such high stability allows SepaxGP-C18 extremely suitable for validation of various analytes.

Selectivity for GP-C18 and the Competitors' C18 Phases

Selectivity is very important for detection of the components of low abundance and impurities from the analysis of pharmaceuticals in the real applications. The comparative tests shown below from the method development of a drug molecule clearly demonstrated that GP-C18 is among the best products for high selectivity and sensitivity in detecting low abundant components and the impurities.

Conditions
Column:	Inertsil ODS-3, 3 µm (Varian) Xterra RP18, 3.5 µm (Waters) Suplex PKB-100, 5 µm (Supelco) Sepax GP-C18, 3 µm
Mobile phase:	A: 99% Boric buffer (5.0 mM, pH 7.0)/1% Acetonitrile B: 40% Boric buffer (5.0 mM, pH 7.0)/60% Acetonitrile
Gradient:	0-3 min (100% A); 3-38min (0-100%B); 38-43 min (100% B)
Column Temperature:	50 °C
Flow rate:	1.0 mL/min
Detection wavelength:	210 nm
Sample:	A real pharmaceutical sample (undisclosed)

GP-C18      1.8 µm         2.2 µm         3 µm         5 µm         10 µm

Sepax GP-C18 Sign In to see pricing Part Number Particle Size Pore Size ID×Length 101181-2103 1.8 µm 120 Å 2.1×30mm 101181-2105 1.8 µm 120 Å 2.1×50mm 101181-2110 1.8 µm 120 Å 2.1×100mm 101181-2115 1.8 µm 120 Å 2.1×150mm 101181-3003 1.8 µm 120 Å 3.0×30mm 101181-3005 1.8 µm 120 Å 3.0×50mm 101181-3010 1.8 µm 120 Å 3.0×100mm 101181-3015 1.8 µm 120 Å 3.0×150mm 101181-4603 1.8 µm 120 Å 4.6×30mm 101181-4605 1.8 µm 120 Å 4.6×50mm 101181-4610 1.8 µm 120 Å 4.6×100mm 101181-4615 1.8 µm 120 Å 4.6×150mm Sepax GP-C18 Sign In to see pricing Part Number Particle Size Pore Size ID×Length 101182-2103 2.2 µm 120 Å 2.1×30mm 101182-2105 2.2 µm 120 Å 2.1×50mm 101182-2110 2.2 µm 120 Å 2.1×100mm 101182-2115 2.2 µm 120 Å 2.1×150mm 101182-3003 2.2 µm 120 Å 3.0×30mm 101182-3005 2.2 µm 120 Å 3.0×50mm 101182-3010 2.2 µm 120 Å 3.0×100mm 101182-3015 2.2 µm 120 Å 3.0×150mm 101182-4603 2.2 µm 120 Å 4.6×30mm 101182-4605 2.2 µm 120 Å 4.6×50mm 101182-4610 2.2 µm 120 Å 4.6×100mm 101182-4615 2.2 µm 120 Å 4.6×150mm Sepax GP-C18 Sign In to see pricing Part Number Particle Size Pore Size ID×Length 101183-2001 [1] 3 µm 120 Å 2.0×10mm 101183-2001C [2] 3 µm 120 Å 2.0×10mm 101183-2103 3 µm 120 Å 2.1×30mm 101183-2105 3 µm 120 Å 2.1×50mm 101183-2110 3 µm 120 Å 2.1×100mm 101183-2115 3 µm 120 Å 2.1×150mm 101183-2125 3 µm 120 Å 2.1×250mm 101183-3003 3 µm 120 Å 3.0×30mm 101183-3005 3 µm 120 Å 3.0×50mm 101183-3010 3 µm 120 Å 3.0×100mm 101183-3015 3 µm 120 Å 3.0×150mm 101183-3025 3 µm 120 Å 3.0×250mm 101183-4001 [1] 3 µm 120 Å 4.0×10mm 101183-4001C [2] 3 µm 120 Å 4.0×10mm 101183-4603 3 µm 120 Å 4.6×30mm 101183-4605 3 µm 120 Å 4.6×50mm 101183-4610 3 µm 120 Å 4.6×100mm 101183-4615 3 µm 120 Å 4.6×150mm 101183-4625 3 µm 120 Å 4.6×250mm [1]: Guard Cartridge only. [2]: Guard Cartridge with holder (Part number ended with "C"). Precolumn Filters (Alternative choice for Guard Cartridge) P/N#: 102000-P355 (2 µm PEEK frit), $65.00/ea     - for column with particle size 5 µm and 10 µm. P/N#: 102000-P356 (0.5 µm PEEK frit), $65.00/ea     - for column with particle size 3 µm, or less than 3 µm. PEEK Refill Frits (For Precolumn Filter) P/N#: 102001-P355, 2 µm, 5 units/pk, $100/pk P/N#: 102001-P356, 0.5 µm, 5 units/pk, $100/pk Sepax GP-C18 Sign In to see pricing Part Number Particle Size Pore Size ID×Length 101185-2001 [1] 5 µm 120 Å 2.0×10mm 101185-2001C [2] 5 µm 120 Å 2.0×10mm 101185-2103 5 µm 120 Å 2.1×30mm 101185-2105 5 µm 120 Å 2.1×50mm 101185-2110 5 µm 120 Å 2.1×100mm 101185-2115 5 µm 120 Å 2.1×150mm 101185-2125 5 µm 120 Å 2.1×250mm 101185-3003 5 µm 120 Å 3.0×30mm 101185-3005 5 µm 120 Å 3.0×50mm 101185-3010 5 µm 120 Å 3.0×100mm 101185-3015 5 µm 120 Å 3.0×150mm 101185-3025 5 µm 120 Å 3.0×250mm 101185-4001 [1] 5 µm 120 Å 4.0×10mm 101185-4001C [2] 5 µm 120 Å 4.0×10mm 101185-4603 5 µm 120 Å 4.6×30mm 101185-4605 5 µm 120 Å 4.6×50mm 101185-4610 5 µm 120 Å 4.6×100mm 101185-4615 5 µm 120 Å 4.6×150mm 101185-4625 5 µm 120 Å 4.6×250mm 101185-7815 5 µm 120 Å 7.8×150mm 101185-7825 5 µm 120 Å 7.8×250mm 101185-10005 5 µm 120 Å 10.0×50mm 101185-10010 5 µm 120 Å 10.0×100mm 101185-10015 5 µm 120 Å 10.0×150mm 101185-10025 5 µm 120 Å 10.0×250mm 101185-21201 [1] 5 µm 120 Å 21.2×10mm 101185-21205 5 µm 120 Å 21.2×50mm 101185-21210 5 µm 120 Å 21.2×100mm 101185-21215 5 µm 120 Å 21.2×150mm 101185-21225 5 µm 120 Å 21.2×250mm 101185-30005 5 µm 120 Å 30.0×50mm 101185-30010 5 µm 120 Å 30.0×100mm 101185-30015 5 µm 120 Å 30.0×150mm 101185-30025 5 µm 120 Å 30.0×250mm [1] Need guard cartridge holder P/N#: 102000-21201 Price: $220.00/EA (for 21.2×10mm guard cartridge) Sepax GP-C18 Sign In to see pricing Part Number Particle Size Pore Size ID×Length 101189-7815 10 µm 120 Å 7.8×150mm 101189-7825 10 µm 120 Å 7.8×250mm 101189-10005 10 µm 120 Å 10.0×50mm 101189-10010 10 µm 120 Å 10.0×100mm 101189-10015 10 µm 120 Å 10.0×150mm 101189-10025 10 µm 120 Å 10.0×250mm 101189-21205 10 µm 120 Å 21.2×50mm 101189-21210 10 µm 120 Å 21.2×100mm 101189-21215 10 µm 120 Å 21.2×150mm 101189-21225 10 µm 120 Å 21.2×250mm 101189-30005 10 µm 120 Å 30.0×50mm 101189-30010 10 µm 120 Å 30.0×100mm 101189-30015 10 µm 120 Å 30.0×150mm 101189-30025 10 µm 120 Å 30.0×250mm

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