Utilizing proprietary surface technologies, Unix SEC-300 phase is made of uniform, hydrophilic, and neutral nanometer thick films chemically bonded to high purity and mechanically stabilized silica with the particle size of 1.8 µm. The combination of small particle size and large pore volume of Unix SEC-300 renders the highest separation efficiency and resolution of analytes. The well-controlled surface chemistry results in excellent lot-to-lot reproducibility. Our unique bonding chemistry, coupled with the maximized bonding density, allows Unix SEC-300 to provide high stability and negligible non-specific interactions. Typical applications for Unix SEC-300 columns include separation and analysis of biological molecules and water-soluble polymers.
Unix SEC Technical Specifications
Neutral, hydrophilic film bonded silica
Average Particle size
Protein MW range (native)
5,000 - 1,250,000 Da
2.0 - 8.5 (pH 8.5-9.5 can be tolerated temporarily.)
Application of proprietary surface modification techniques to Unix™ SEC-300 resin produces a densely bonded silica surface, which greatly hinders the diffusion of molecules that would attack the bond between silica surface and tethered moieties, thus enabling high stability over a wide range of pH from 2.0 to 8.5.
Unix™ SEC-300 phase is compatible with most aqueous buffers, such as ammonium acetate, phosphate, trizma and so on. Unix™ SEC-300 phase can tolerate high concentration of salts. Furthermore, Unix™ SEC columns are stable in both organic solvents, such as methanol, ethanol, THF, DMF, DMSO, etc. as well as mixture of water and organic solvents.
The controlled surface chemistry used to synthesize Unix™ SEC-300 phase makes the surface coating highly reproducible, leading to consistent column manufacturing. Separation variation from batch-to-batch is controlled to be within 5% for retention time. Figure 5 demonstrates the lot-to-lot variation of three different Unix™ SEC-300 lots.
Figure 5. Unix™ Lot-to-Lot Reproducibility on Protein Standards