Non-porous Proteomix Ion-exchange Phases - High Resolution and High Capacity
Proteomix ion-exchange phases have been innovatively developed for achieving high ion-exchange capacity, a breakthrough technology for non-porous resins. Proteomix packing support is composed of a rigid, spherical, highly cross-linked poly(styrene divinylbenzene) (PS/DVB) non-porous bead with the particle size selections of 1, 1.7, 3, 5 and 10 µm. The PS/DVB resin surface is grafted with a highly hydrophilic, neutral polymer thin layer with the thickness in the range of nanometer. The hydrophobic PS/DVB resin surface is totally covered by such a hydrophilic coating that eliminates non-specific bindings with biological analytes, leading to high efficiency and high recovery separations for biological molecules. On the top of the hydrophilic layer, ion-exchange functional groups are attached via a proprietary chemistry, resulting in high capacity ion-exchange layer.
The uniqueness of non-porous Proteomix ion-exchange phases is that they combine increased capacity and resolution with their intrinsic advantages of high efficiency and high separation speed. Proteomics ion-exchange phases are especially suitable for high resolution, high efficiency and high recovery separations of proteins, oligonucleotides, peptides, polysaccharides, cell lysates, nanoparticles and nanotubes.
Porous Proteomix Ion-exchange Phases
Porous Proteomix packing support is composed of a rigid, spherical, highly cross-linked poly(styrene divinylbenzene) (PS/DVB) particle with a pore size of 500 Å. The surface is grafted with a highly hydrophilic, neutral polymer thin layer, and further chemically bonded with a layer of ion-exchange functional groups. Four ion-exchange phases (SCX, WCX, SAX and WAX) are specially designed for high capacity and high recovery separations of proteins, oligonucleotides, carbohydrates, and peptides. The porous Proteomix resins have particle size of 5 and 10 µm for analytical separation, and 30 and 50 µm for preparative separation.
The chemical structure of Proteomix ion-exchange phases is composed of a rigid PS/DVB core, a densely packed, nanometer thick, hydrophilic coating, and a uniform ion-exchange layer, as shown in Figure 1.
Figure 1. Schematic illustration of the chemical structure of Proteomix ion-exchange phases.
As shown in Figure 2, Proteomix ion-exchange phases are composed of SCX, WCX, SAX, and WAX. The Proteomix SCX phase is a strong cation exchanger with sulfonate functional groups chemically bonded to the top of the hydrophilic coating for both porous and non-porous PS/DVB resins. The Proteomix WCX phase is a weak cation exchanger with carboxylate functional groups chemically bonded to the top of the hydrophilic coating for both porous and non-porous PS/DVB resins. The Proteomix SAX phase is a strong anion exchanger with quaternary ammonium functional groups chemically bonded to the top of the hydrophilic coating for both porous and non-porous PS/DVB resins. The Proteomix WAX phase is a weak anion exchanger with tertiary amine functional groups chemically bonded to the top of the hydrophilic coating for both porous and non-porous PS/DVB resins.
Figure 2.
Chemical compositions of Proteomix SCX, WAX, SAX, and WAX phases.
Technical Specifications
| Products | Pore Size | Particle Size | Dynamic Binding Capacity | pH range |
|---|
| Proteomix SCX-NP1.7,NP3,NP5,NP10 |
Non-porous |
1.7,3,5,10 µm |
~60,54,38,and 20 mg/mL |
2-12 |
| Proteomix SCX-POR10 |
500 Å |
10 µm |
~35 mg/mL |
2-12 |
| Proteomix WCX-NP1.7,NP3,NP5,NP10 |
Non-porous |
1.7,3,5,10 µm |
~25,19,15,and 10 mg/mL |
2-12 |
| Proteomix WCX-POR10 |
500 Å |
10 µm |
~22 mg/mL |
2-12 |
| Proteomix SAX-NP1.7,NP3,NP5,NP10 |
Non-porous |
1.7,3,5,10 µm |
~43,35,28,and 17 mg/mL |
2-12 |
| Proteomix SAX-POR10 |
500 Å |
10 µm |
~25 mg/mL |
2-12 |
| Proteomix WAX-NP1.7,NP3,NP5,NP10 |
Non-porous |
1.7,3,5,10 µm |
~35,26,18,and 12 mg/mL |
2-12 |
| Proteomix WAX-POR10 |
500 Å |
10 µm |
~20 mg/mL |
2-12 |
Separation of a protein mixture by a Proteomix SCX-NP3 column (3 µm, 4.6×50 mm). Eluent: 10 mM phosphate, pH 6.0; Gradient: 0-1.0 M NaCl (15 min); Flow rate: 0.5 mL/min; Detection (UV): 280 nm.
Figure above is a typical test chromatogram for a Proteomix SCX-NP column (3 µm). Extremely high efficiency separation was achieved for 5 proteins. Those proteins were eluted based on their charge state (pI) from low to high: ovalbumin, ribonuclease A, cytochrome C, aprotinin, and lysozyme. At the separation conditions (pH 6.0), ovalbumin is negatively charged and eluted at the void volume, indicating no non-specific bindings.
- Uniform polymer beads as the packing support
- Proprietary surface chemistry specially designed for elimination of non-specific bindings
- Unprecedented separation efficiency, selectivity and resolving power
- Tunable separation capacity with combination of the pore structure and the controllable surface chemistry
- Complete selection for analytical, semi-preparative and preparative separations
- Wide particle size selection
- High stability
- High recovery
- Well suited for UPLC system
Technology Advancements for the Proeteomix Non-porous Ion-exchanger
High Capacity of the Proteomix Non-Porous Resins
It is well known that the non-porous resin maximizes the mass transfer and minimizes the lateral diffusion, resulting in high speed and high efficiency separations. However, due to the low surface area, the separation capacity is low. The low capacity issue creates a great challenge for many applications for the non-porous resins. The Proteomix ion-exchange resins developed by the Sepax proprietary technologies have a breakthrough on the improvement of their capacities for the non-porous resins. This new technology enables the capacity of the non-porous resins increased to the comparable level to that of the porous resins. Figure 3 shows examples of dynamic binding capacities of the 3 µm Proteomix SAX and WAX non-porous ion-exchange resins: 35 and 26 mg/mL, respectively. Figure 4 shows an example of the dynamic binding capacity of the 3 µm Proteomix strong cation exchange non-porous resin. Its dynamic binding capacity reaches as high as 53.5 mg/mL.
Figure 3. The dynamic binding capacity tests for the Proteomix anion-exchange resins (3 µm) by flowing BSA into the columns with the running conditions: 10 mM Tris/HCl, pH 8.0; 3 mg/mL BSA; flow rate: 0.25 mL/min; detection: 280 nm.
Figure 4. The dynamic binding capacity test for the Proteomix SCX resin (3 µm) by flowing lysozyme into the column with the running conditions: 10 mM Tris/HCl, pH 8.0; 3 mg/mL BSA; flow rate: 0.25 mL/min; detection: 280 nm.
High dynamic binding capacity leads to high sample loading without deterioration of the separation efficiency and the resolution. Figure 5 shows the elution of cytochrome C with the loading up to 200 mg for a 1.7 µm Proteomix SCX non-porous resin without any deterioration of the separation.
Figure 5. Elution of cytochrome C (20 mg/mL) with the conditions: A, 20 mM PBS; B, A+1.0 M NaCl; 0-70%B (21 min); 0.35 mL/min, 2,800psi; UV 280 nm.
High Separation Efficiency, Resolution and Selectivity
The Proteomix SCX-NP, WCX-NP, SAX-NP, and WAX-NP resins are based on a nonporous PS/DVB particle that is coated with a proprietary hydrophilic layer and functionalized with a uniform ion-exchange layer.
Those phases have three unique features. First, the nanometer thick hydrophilic layer completely eliminates the non-specific interactions with the biological analytes. Second, non-porous beads minimize the biological analytes¡¯ lateral diffusion and suppress their diffusion into the chromatographic bed. Third, Sepax¡¯s proprietary technology synthesizes a uniform and densely packed layer of ion-exchange functional groups. Such uniquely designed Proteomix SCX-NP, WCX-NP, SAX-NP, and WAX-NP phases offer the highest resolution and efficiency separations for proteins, oligonucleotides, carbohydrates, and peptides. Figure 6 is a typical test chromatogram for separation of three proteins: ribonuclease A, cytochrome C, and lysozyme by a 4.6×50 mm, Proteomix SCX-NP column (3 µm). The efficiency of lysozyme reaches 100,000 of plates with 5 cm long column. Such a high efficiency separation is unprecedented.
Figure 6. Separation of a protein mixture by a Proteomix SCX-NP column.
The unique the Proteomix SCX-NP, WCX-NP, SAX-NP, and WAX-NP phases offer highest selectivity for protein separations. Figure 7 shows an elution profile of lysozyme by a Proteomix SCX-NP3 phase (3 µm). Six peaks are well resolved with a short column (4.6×50 mm). Such a high selectivity and resolving power is unmatched with any other competitors.
Figure 7. The elution profile of lysozyme from its multiple impurities by a Proteomix SCX-NP column (4.6x50mm, 3 mm). The separation conditions are the same as those in Figure 6.
Figure 8 is a typical test chromatogram for a 5 mm, Proteomix SAX-NP column for separation of a mixture of ovalbumin and BSA. The high resolution and high selectivity of the Proteomix SAX-NP phase can well separate the impurities contained in the ovalbumin mixture, as well as the BSA dimer from BSA.
Figure 8. Elution profile of a mixture of ovalbumin and BSA by a designs of Proteomix SAX-NP5 column.
Particle Size Impact on the Separation
The smaller the particle size, the better separation efficiency and higher separation speed. The Proteomix ion-exchange resins have a wide range of the particle sizes from 1.0 to 10 µm for analytical and semi-preparative separation applications. Figure 8 is the separation of three proteins by four weak cation exchangers with the particle size of 1.7, 3, 5, and 10 µm, separately. It is clearly seen that the smaller particle generates higher efficiency and resolution. One of the impurities in lysozyme labeled by Peak N is barely separate with the 10 µm WCX column. For 5 µm WCX exchanger, it is almost a baseline separation. When the particle size is decreased to 3 µm, it becomes a baseline separation. With the particle size further decreased to 1.7 µm, the impurity is well separated.
Figure 9. Separation of a protein mixture by the Proteomix WCX-NP columns with the particle size varying from 1.7 to 10 µm with the separation conditions: A, 20 mM PBS, B, A+1.0 M NaCl, 0-100%B (20 min); flow rate: 1.0 mL/min, 0.75 mL/min (WCX-NP1.7); proteins (1.0 mg/mL): (1) Ribonuclease A, (2) Cytochrome C, and (3) Lysozyme; UV 280 nm.
High Lot to Lot Reproducibility
With well controlled polymer resin production and the surface chemistry, the manufacturing of the Proteomix ion-exchangers is highly reproducible. The typical variation of the retention time is less than 6% from batch to batch. One example is shown in Figure 11 for the production of three lots of 3 µm Proteomix SAX-NP anion-exchanger.
Figure 11. The reproducibility of three lots of the Proteomix SAX resins with the test conditions: SAX-NP3 columns (3 µm, 4.6×50 mm); 20 mM Tris, pH 8.0, 0-0.3 M NaCl (15 min); flow rate at 0.5 mL/min; UV 214.
The Porous Protomix Ion-Exchanger
The Proteomix SCX-POR, WCX-POR, SAX-POR, and WAX-POR resins are based on porous PS/DVB resins that are coated with a proprietary hydrophilic layer and functionalized with a uniform ion-exchange layer. These phases have three unique features. First, the nanometer thick hydrophilic layer completely eliminates the non-specific interactions with biological analytes. Second, Sepax¡¯s proprietary technology synthesizes a uniform and densely packed layer of ion-exchange functional groups. Third, porous beads provide high surface area, leading to high capacity separation. Such uniquely designed Proteomix SCX-POR, WCX-POR, SAX-POR, and WAX-POR phases offer high resolution and high capacity separations for proteins, oligonucleotides and peptides. The Proteomix SCX-POR, WCX-POR, SAX-POR, and WAX-POR phases are well suited for semi-preparative and preparative separation and purification of biomolecules.
The Proteomix SCX, WCX, SAX, and WAX columns are manufactured and tested under the strictest specifications, resulting in high column-to-column and lot-to-lot reproducibility.
Column Dimension Availability
The column dimensions of the Proteomix SCX, WCX, SAX, and WAX products are 0.75, 2.1, 3.0, 4.6, 7.8, 10, and 21.2 mm I.D., and 2, 3, 5, 10, 15, 25, and 30 cm length. Sepax also offers custom-made columns.
Operating Conditions
Temperature limit
80°C.
Operating pressure limit
4,000 psi for 10 µm resins;
6,000 psi for 5 µm resins;
8,000 psi for 3 µm resins;
12,000 psi for 1.7 µm resins.
Flow rate
Typically 0.5 and 1.0 mL/min for a 2.1 and 4.6 mm I.D. column.
Mobile phase compatibility
The Proteomix columns are compatible with both aqueous solution, and a mixture of water and organic solvents, such as acetonitrile, methanol. Typical buffers include phosphate for SCX and WCX ion-exchangers, and tris for SAX and WAX ion-exchangers.
The Proteomix SAX and WAX columns are compatible with nonionic and zwitterionic detergents. The Proteomix SAX and WAX columns are incompatible with anionic detergents. The Proteomix SCX and WCX columns are compatible with nonionic and zwitterionic detergents. The Proteomix SCX and WCX columns are incompatible with cationic detergents.
Sepax Proteomix SCX-NP | Part Number | Particle Size | Pore Size | ID×Length | Price |
|---|
| | 401NP2-2001 * | 1.7 µm | Non-Porous | 2.0×10mm | $425.00 | | 401NP2-2103 | 1.7 µm | Non-Porous | 2.1×30mm | $900.00 | | 401NP2-2105 | 1.7 µm | Non-Porous | 2.1×50mm | $950.00 | | 401NP2-2110 | 1.7 µm | Non-Porous | 2.1×100mm | $1,095.00 | | 401NP2-3003 | 1.7 µm | Non-Porous | 3.0×30mm | $950.00 | | 401NP2-3005 | 1.7 µm | Non-Porous | 3.0×50mm | $1,025.00 | | 401NP2-3010 | 1.7 µm | Non-Porous | 3.0×100mm | $1,150.00 | | 401NP2-4001 * | 1.7 µm | Non-Porous | 4.0×10mm | $425.00 | | 401NP2-4603 | 1.7 µm | Non-Porous | 4.6×30mm | $975.00 | | 401NP2-4605 | 1.7 µm | Non-Porous | 4.6×50mm | $1,050.00 | | 401NP2-4610 | 1.7 µm | Non-Porous | 4.6×100mm | $1,250.00 | | 401NP2-4615 | 1.7 µm | Non-Porous | 4.6×150mm | $1,350.00 | | 401NP3-2001 * | 3 µm | Non-Porous | 2.0×10mm | $375.00 | | 401NP3-2103 | 3 µm | Non-Porous | 2.1×30mm | $900.00 | | 401NP3-2105 | 3 µm | Non-Porous | 2.1×50mm | $950.00 | | 401NP3-2110 | 3 µm | Non-Porous | 2.1×100mm | $1,095.00 | | 401NP3-3003 | 3 µm | Non-Porous | 3.0×30mm | $900.00 | | 401NP3-3005 | 3 µm | Non-Porous | 3.0×50mm | $950.00 | | 401NP3-3010 | 3 µm | Non-Porous | 3.0×100mm | $1,095.00 | | 401NP3-4001 * | 3 µm | Non-Porous | 4.0×10mm | $375.00 | | 401NP3-4603 | 3 µm | Non-Porous | 4.6×30mm | $900.00 | | 401NP3-4605 | 3 µm | Non-Porous | 4.6×50mm | $950.00 | | 401NP3-4610 | 3 µm | Non-Porous | 4.6×100mm | $1,095.00 | | 401NP3-4615 | 3 µm | Non-Porous | 4.6×150mm | $1,195.00 | | 401NP5-2001 * | 5 µm | Non-Porous | 2.0×10mm | $325.00 | | 401NP5-2103 | 5 µm | Non-Porous | 2.1×30mm | $850.00 | | 401NP5-2105 | 5 µm | Non-Porous | 2.1×50mm | $950.00 | | 401NP5-2110 | 5 µm | Non-Porous | 2.1×100mm | $1,050.00 | | 401NP5-2115 | 5 µm | Non-Porous | 2.1×150mm | $1,150.00 | | 401NP5-4001 * | 5 µm | Non-Porous | 4.0×10mm | $325.00 | | 401NP5-4603 | 5 µm | Non-Porous | 4.6×30mm | $875.00 | | 401NP5-4605 | 5 µm | Non-Porous | 4.6×50mm | $950.00 | | 401NP5-4610 | 5 µm | Non-Porous | 4.6×100mm | $1,050.00 | | 401NP5-4615 | 5 µm | Non-Porous | 4.6×150mm | $1,150.00 | | 401NP5-4625 | 5 µm | Non-Porous | 4.6×250mm | $1,250.00 | | 401NP10-2001 * | 10 µm | Non-Porous | 2.0×10mm | $275.00 | | 401NP10-2103 | 10 µm | Non-Porous | 2.1×30mm | $375.00 | | 401NP10-2105 | 10 µm | Non-Porous | 2.1×50mm | $475.00 | | 401NP10-2110 | 10 µm | Non-Porous | 2.1×100mm | $755.00 | | 401NP10-2115 | 10 µm | Non-Porous | 2.1×150mm | $975.00 | | 401NP10-4001 * | 10 µm | Non-Porous | 4.0×10mm | $275.00 | | 401NP10-4603 | 10 µm | Non-Porous | 4.6×30mm | $375.00 | | 401NP10-4605 | 10 µm | Non-Porous | 4.6×50mm | $475.00 | | 401NP10-4610 | 10 µm | Non-Porous | 4.6×100mm | $755.00 | | 401NP10-4615 | 10 µm | Non-Porous | 4.6×150mm | $975.00 | | 401NP10-4625 | 10 µm | Non-Porous | 4.6×250mm | $1,050.00 |
* Guard cartridge holder needed.
Guard cartridge holder
P/N#: 102000-2001, $55.00/EA, for 2.0×10mm guard cartridge
P/N#: 102000-4001, $55.00/EA, for 4.0×10mm guard cartridge
Sepax Proteomix WCX-NP | Part Number | Particle Size | Pore Size | ID×Length | Price |
|---|
| | 402NP2-2001 * | 1.7 µm | Non-Porous | 2.0×10mm | $425.00 | | 402NP2-2103 | 1.7 µm | Non-Porous | 2.1×30mm | $900.00 | | 402NP2-2105 | 1.7 µm | Non-Porous | 2.1×50mm | $950.00 | | 402NP2-2110 | 1.7 µm | Non-Porous | 2.1×100mm | $1,095.00 | | 402NP2-3003 | 1.7 µm | Non-Porous | 3.0×30mm | $950.00 | | 402NP2-3005 | 1.7 µm | Non-Porous | 3.0×50mm | $1,025.00 | | 402NP2-3010 | 1.7 µm | Non-Porous | 3.0×100mm | $1,150.00 | | 402NP2-4001 * | 1.7 µm | Non-Porous | 4.0×10mm | $425.00 | | 402NP2-4603 | 1.7 µm | Non-Porous | 4.6×30mm | $975.00 | | 402NP2-4605 | 1.7 µm | Non-Porous | 4.6×50mm | $1,050.00 | | 402NP2-4610 | 1.7 µm | Non-Porous | 4.6×100mm | $1,250.00 | | 402NP2-4615 | 1.7 µm | Non-Porous | 4.6×150mm | $1,350.00 | | 402NP3-2001 * | 3 µm | Non-Porous | 2.0×10mm | $375.00 | | 402NP3-2103 | 3 µm | Non-Porous | 2.1×30mm | $900.00 | | 402NP3-2105 | 3 µm | Non-Porous | 2.1×50mm | $950.00 | | 402NP3-2110 | 3 µm | Non-Porous | 2.1×100mm | $1,095.00 | | 402NP3-3003 | 3 µm | Non-Porous | 3.0×30mm | $900.00 | | 402NP3-3005 | 3 µm | Non-Porous | 3.0×50mm | $950.00 | | 402NP3-3010 | 3 µm | Non-Porous | 3.0×100mm | $1,095.00 | | 402NP3-4001 * | 3 µm | Non-Porous | 4.0×10mm | $375.00 | | 402NP3-4603 | 3 µm | Non-Porous | 4.6×30mm | $900.00 | | 402NP3-4605 | 3 µm | Non-Porous | 4.6×50mm | $950.00 | | 402NP3-4610 | 3 µm | Non-Porous | 4.6×100mm | $1,095.00 | | 402NP3-4615 | 3 µm | Non-Porous | 4.6×150mm | $1,195.00 | | 402NP5-2001 * | 5 µm | Non-Porous | 2.0×10mm | $325.00 | | 402NP5-2103 | 5 µm | Non-Porous | 2.1×30mm | $850.00 | | 402NP5-2105 | 5 µm | Non-Porous | 2.1×50mm | $950.00 | | 402NP5-2110 | 5 µm | Non-Porous | 2.1×100mm | $1,050.00 | | 402NP5-2115 | 5 µm | Non-Porous | 2.1×150mm | $1,150.00 | | 402NP5-4001 * | 5 µm | Non-Porous | 4.0×10mm | $325.00 | | 402NP5-4603 | 5 µm | Non-Porous | 4.6×30mm | $875.00 | | 402NP5-4605 | 5 µm | Non-Porous | 4.6×50mm | $950.00 | | 402NP5-4610 | 5 µm | Non-Porous | 4.6×100mm | $1,050.00 | | 402NP5-4615 | 5 µm | Non-Porous | 4.6×150mm | $1,150.00 | | 402NP5-4625 | 5 µm | Non-Porous | 4.6×250mm | $1,250.00 | | 402NP10-2001 * | 10 µm | Non-Porous | 2.0×10mm | $275.00 | | 402NP10-2103 | 10 µm | Non-Porous | 2.1×30mm | $375.00 | | 402NP10-2105 | 10 µm | Non-Porous | 2.1×50mm | $475.00 | | 402NP10-2110 | 10 µm | Non-Porous | 2.1×100mm | $755.00 | | 402NP10-2115 | 10 µm | Non-Porous | 2.1×150mm | $975.00 | | 402NP10-4001 * | 10 µm | Non-Porous | 4.0×10mm | $275.00 | | 402NP10-4603 | 10 µm | Non-Porous | 4.6×30mm | $375.00 | | 402NP10-4605 | 10 µm | Non-Porous | 4.6×50mm | $475.00 | | 402NP10-4610 | 10 µm | Non-Porous | 4.6×100mm | $755.00 | | 402NP10-4615 | 10 µm | Non-Porous | 4.6×150mm | $975.00 | | 402NP10-4625 | 10 µm | Non-Porous | 4.6×250mm | $1,050.00 |
* Guard cartridge holder needed.
Guard cartridge holder
P/N#: 102000-2001, $55.00/EA, for 2.0×10mm guard cartridge
P/N#: 102000-4001, $55.00/EA, for 4.0×10mm guard cartridge
Sepax Proteomix SAX-NP | Part Number | Particle Size | Pore Size | ID×Length | Price |
|---|
| | 403NP2-2001 * | 1.7 µm | Non-Porous | 2.0×10mm | $425.00 | | 403NP2-2103 | 1.7 µm | Non-Porous | 2.1×30mm | $900.00 | | 403NP2-2105 | 1.7 µm | Non-Porous | 2.1×50mm | $950.00 | | 403NP2-2110 | 1.7 µm | Non-Porous | 2.1×100mm | $1,095.00 | | 403NP2-3003 | 1.7 µm | Non-Porous | 3.0×30mm | $950.00 | | 403NP2-3005 | 1.7 µm | Non-Porous | 3.0×50mm | $1,025.00 | | 403NP2-3010 | 1.7 µm | Non-Porous | 3.0×100mm | $1,150.00 | | 403NP2-4001 * | 1.7 µm | Non-Porous | 4.0×10mm | $425.00 | | 403NP2-4603 | 1.7 µm | Non-Porous | 4.6×30mm | $975.00 | | 403NP2-4605 | 1.7 µm | Non-Porous | 4.6×50mm | $1,050.00 | | 403NP2-4610 | 1.7 µm | Non-Porous | 4.6×100mm | $1,250.00 | | 403NP2-4615 | 1.7 µm | Non-Porous | 4.6×150mm | $1,350.00 | | 403NP3-2001 * | 3 µm | Non-Porous | 2.0×10mm | $375.00 | | 403NP3-2103 | 3 µm | Non-Porous | 2.1×30mm | $900.00 | | 403NP3-2105 | 3 µm | Non-Porous | 2.1×50mm | $950.00 | | 403NP3-2110 | 3 µm | Non-Porous | 2.1×100mm | $1,095.00 | | 403NP3-3003 | 3 µm | Non-Porous | 3.0×30mm | $900.00 | | 403NP3-3005 | 3 µm | Non-Porous | 3.0×50mm | $950.00 | | 403NP3-3010 | 3 µm | Non-Porous | 3.0×100mm | $1,095.00 | | 403NP3-4001 * | 3 µm | Non-Porous | 4.0×10mm | $375.00 | | 403NP3-4603 | 3 µm | Non-Porous | 4.6×30mm | $900.00 | | 403NP3-4605 | 3 µm | Non-Porous | 4.6×50mm | $950.00 | | 403NP3-4610 | 3 µm | Non-Porous | 4.6×100mm | $1,095.00 | | 403NP3-4615 | 3 µm | Non-Porous | 4.6×150mm | $1,195.00 | | 403NP5-2001 * | 5 µm | Non-Porous | 2.0×10mm | $325.00 | | 403NP5-2103 | 5 µm | Non-Porous | 2.1×30mm | $850.00 | | 403NP5-2105 | 5 µm | Non-Porous | 2.1×50mm | $950.00 | | 403NP5-2110 | 5 µm | Non-Porous | 2.1×100mm | $1,050.00 | | 403NP5-2115 | 5 µm | Non-Porous | 2.1×150mm | $1,150.00 | | 403NP5-4001 * | 5 µm | Non-Porous | 4.0×10mm | $325.00 | | 403NP5-4603 | 5 µm | Non-Porous | 4.6×30mm | $875.00 | | 403NP5-4605 | 5 µm | Non-Porous | 4.6×50mm | $950.00 | | 403NP5-4610 | 5 µm | Non-Porous | 4.6×100mm | $1,050.00 | | 403NP5-4615 | 5 µm | Non-Porous | 4.6×150mm | $1,150.00 | | 403NP5-4625 | 5 µm | Non-Porous | 4.6×250mm | $1,250.00 | | 403NP10-2001 * | 10 µm | Non-Porous | 2.0×10mm | $275.00 | | 403NP10-2103 | 10 µm | Non-Porous | 2.1×30mm | $375.00 | | 403NP10-2105 | 10 µm | Non-Porous | 2.1×50mm | $475.00 | | 403NP10-2110 | 10 µm | Non-Porous | 2.1×100mm | $755.00 | | 403NP10-2115 | 10 µm | Non-Porous | 2.1×150mm | $975.00 | | 403NP10-4001 * | 10 µm | Non-Porous | 4.0×10mm | $275.00 | | 403NP10-4603 | 10 µm | Non-Porous | 4.6×30mm | $375.00 | | 403NP10-4605 | 10 µm | Non-Porous | 4.6×50mm | $475.00 | | 403NP10-4610 | 10 µm | Non-Porous | 4.6×100mm | $755.00 | | 403NP10-4615 | 10 µm | Non-Porous | 4.6×150mm | $975.00 | | 403NP10-4625 | 10 µm | Non-Porous | 4.6×250mm | $1,050.00 |
* Guard cartridge holder needed.
Guard cartridge holder
P/N#: 102000-2001, $55.00/EA, for 2.0×10mm guard cartridge
P/N#: 102000-4001, $55.00/EA, for 4.0×10mm guard cartridge
Sepax Proteomix WAX-NP | Part Number | Particle Size | Pore Size | ID×Length | Price |
|---|
| | 404NP2-2001 * | 1.7 µm | Non-Porous | 2.0×10mm | $425.00 | | 404NP2-2103 | 1.7 µm | Non-Porous | 2.1×30mm | $900.00 | | 404NP2-2105 | 1.7 µm | Non-Porous | 2.1×50mm | $950.00 | | 404NP2-2110 | 1.7 µm | Non-Porous | 2.1×100mm | $1,095.00 | | 404NP2-3003 | 1.7 µm | Non-Porous | 3.0×30mm | $950.00 | | 404NP2-3005 | 1.7 µm | Non-Porous | 3.0×50mm | $1,025.00 | | 404NP2-3010 | 1.7 µm | Non-Porous | 3.0×100mm | $1,150.00 | | 404NP2-4001 * | 1.7 µm | Non-Porous | 4.0×10mm | $425.00 | | 404NP2-4603 | 1.7 µm | Non-Porous | 4.6×30mm | $975.00 | | 404NP2-4605 | 1.7 µm | Non-Porous | 4.6×50mm | $1,050.00 | | 404NP2-4610 | 1.7 µm | Non-Porous | 4.6×100mm | $1,250.00 | | 404NP2-4615 | 1.7 µm | Non-Porous | 4.6×150mm | $1,350.00 | | 404NP3-2001 * | 3 µm | Non-Porous | 2.0×10mm | $375.00 | | 404NP3-2103 | 3 µm | Non-Porous | 2.1×30mm | $900.00 | | 404NP3-2105 | 3 µm | Non-Porous | 2.1×50mm | $950.00 | | 404NP3-2110 | 3 µm | Non-Porous | 2.1×100mm | $1,095.00 | | 404NP3-3003 | 3 µm | Non-Porous | 3.0×30mm | $900.00 | | 404NP3-3005 | 3 µm | Non-Porous | 3.0×50mm | $950.00 | | 404NP3-3010 | 3 µm | Non-Porous | 3.0×100mm | $1,095.00 | | 404NP3-4001 * | 3 µm | Non-Porous | 4.0×10mm | $375.00 | | 404NP3-4603 | 3 µm | Non-Porous | 4.6×30mm | $900.00 | | 404NP3-4605 | 3 µm | Non-Porous | 4.6×50mm | $950.00 | | 404NP3-4610 | 3 µm | Non-Porous | 4.6×100mm | $1,095.00 | | 404NP3-4615 | 3 µm | Non-Porous | 4.6×150mm | $1,195.00 | | 404NP5-2001 * | 5 µm | Non-Porous | 2.0×10mm | $325.00 | | 404NP5-2103 | 5 µm | Non-Porous | 2.1×30mm | $850.00 | | 404NP5-2105 | 5 µm | Non-Porous | 2.1×50mm | $950.00 | | 404NP5-2110 | 5 µm | Non-Porous | 2.1×100mm | $1,050.00 | | 404NP5-2115 | 5 µm | Non-Porous | 2.1×150mm | $1,150.00 | | 404NP5-4001 * | 5 µm | Non-Porous | 4.0×10mm | $325.00 | | 404NP5-4603 | 5 µm | Non-Porous | 4.6×30mm | $875.00 | | 404NP5-4605 | 5 µm | Non-Porous | 4.6×50mm | $950.00 | | 404NP5-4610 | 5 µm | Non-Porous | 4.6×100mm | $1,050.00 | | 404NP5-4615 | 5 µm | Non-Porous | 4.6×150mm | $1,150.00 | | 404NP5-4625 | 5 µm | Non-Porous | 4.6×250mm | $1,250.00 | | 404NP10-2001 * | 10 µm | Non-Porous | 2.0×10mm | $275.00 | | 404NP10-2103 | 10 µm | Non-Porous | 2.1×30mm | $375.00 | | 404NP10-2105 | 10 µm | Non-Porous | 2.1×50mm | $475.00 | | 404NP10-2110 | 10 µm | Non-Porous | 2.1×100mm | $755.00 | | 404NP10-2115 | 10 µm | Non-Porous | 2.1×150mm | $975.00 | | 404NP10-4001 * | 10 µm | Non-Porous | 4.0×10mm | $275.00 | | 404NP10-4603 | 10 µm | Non-Porous | 4.6×30mm | $375.00 | | 404NP10-4605 | 10 µm | Non-Porous | 4.6×50mm | $475.00 | | 404NP10-4610 | 10 µm | Non-Porous | 4.6×100mm | $755.00 | | 404NP10-4615 | 10 µm | Non-Porous | 4.6×150mm | $975.00 | | 404NP10-4625 | 10 µm | Non-Porous | 4.6×250mm | $1,050.00 |
* Guard cartridge holder needed.
Guard cartridge holder
P/N#: 102000-2001, $55.00/EA, for 2.0×10mm guard cartridge
P/N#: 102000-4001, $55.00/EA, for 4.0×10mm guard cartridge
Sepax Proteomix SCX-NP | Part Number | Particle Size | Pore Size | ID×Length | Price |
|---|
| | 401NP5-7810 | 5 µm | Non-Porous | 7.8×100mm | $1,275.00 | | 401NP5-7825 | 5 µm | Non-Porous | 7.8×250mm | $2,150.00 | | 401NP5-10025 | 5 µm | Non-Porous | 10.0×250mm | Inquire | | 401NP10-7815 | 10 µm | Non-Porous | 7.8×150mm | $1,050.00 | | 401NP10-7825 | 10 µm | Non-Porous | 7.8×250mm | $1,750.00 | | 401NP10-10025 | 10 µm | Non-Porous | 10.0×250mm | Inquire | | 401NP10-21215 | 10 µm | Non-Porous | 21.2×150mm | Inquire | | 401NP10-21225 | 10 µm | Non-Porous | 21.2×250mm | Inquire |
Sepax Proteomix WCX-NP | Part Number | Particle Size | Pore Size | ID×Length | Price |
|---|
| | 402NP5-7810 | 5 µm | Non-Porous | 7.8×100mm | $1,275.00 | | 402NP5-7825 | 5 µm | Non-Porous | 7.8×250mm | $2,150.00 | | 402NP5-10025 | 5 µm | Non-Porous | 10.0×250mm | Inquire | | 402NP10-7815 | 10 µm | Non-Porous | 7.8×150mm | $1,050.00 | | 402NP10-7825 | 10 µm | Non-Porous | 7.8×250mm | $1,750.00 | | 402NP10-10025 | 10 µm | Non-Porous | 10.0×250mm | Inquire | | 402NP10-21215 | 10 µm | Non-Porous | 21.2×150mm | Inquire | | 402NP10-21225 | 10 µm | Non-Porous | 21.2×250mm | Inquire |
Sepax Proteomix SAX-NP | Part Number | Particle Size | Pore Size | ID×Length | Price |
|---|
| | 403NP5-7810 | 5 µm | Non-Porous | 7.8×100mm | $1,275.00 | | 403NP5-7825 | 5 µm | Non-Porous | 7.8×250mm | $2,150.00 | | 403NP5-10025 | 5 µm | Non-Porous | 10.0×250mm | Inquire | | 403NP10-7815 | 10 µm | Non-Porous | 7.8×150mm | $1,050.00 | | 403NP10-7825 | 10 µm | Non-Porous | 7.8×250mm | $1,750.00 | | 403NP10-10025 | 10 µm | Non-Porous | 10.0×250mm | Inquire | | 403NP10-21215 | 10 µm | Non-Porous | 21.2×150mm | Inquire | | 403NP10-21225 | 10 µm | Non-Porous | 21.2×250mm | Inquire |
Sepax Proteomix WAX-NP | Part Number | Particle Size | Pore Size | ID×Length | Price |
|---|
| | 404NP5-7810 | 5 µm | Non-Porous | 7.8×100mm | $1,275.00 | | 404NP5-7825 | 5 µm | Non-Porous | 7.8×250mm | $2,150.00 | | 404NP5-10025 | 5 µm | Non-Porous | 10.0×250mm | Inquire | | 404NP10-7815 | 10 µm | Non-Porous | 7.8×150mm | $1,050.00 | | 404NP10-7825 | 10 µm | Non-Porous | 7.8×250mm | $1,750.00 | | 404NP10-10025 | 10 µm | Non-Porous | 10.0×250mm | Inquire | | 404NP10-21215 | 10 µm | Non-Porous | 21.2×150mm | Inquire | | 404NP10-21225 | 10 µm | Non-Porous | 21.2×250mm | Inquire |
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Proteomix SCX-NP Proteomix WCX-NP Proteomix SAX-NP Proteomix WAX-NP
Proteomix SCX-NP Proteomix WCX-NP Proteomix SAX-NP Proteomix WAX-NP
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Analytical LC Columns
