Sepax Technologies, Inc.

Sepax Antibodix Columns

Description

Packing

Characteristics

Application

Order Info

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Antibodix columns are specially designed for high resolution, high efficiency and high recovery separations of antibodies. The packing support is composed of a rigid, spherical, highly cross-linked poly(styrene divinylbenzene) (PS/DVB) non-porous bead. The non-porous resin has particle size of 1.7, 3, 5 and 10 µm. The PS/DVB resin surface is grafted with a highly hydrophilic, neutral polymer thin layer with the thickness in the range of nanometer. On the top of the hydrophilic layer, weak cation-exchange functional groups are attached via a proprietary chemistry, resulting in high capacity ion-exchange layer.

Highly cross-linked non-porous PS/DVB particle as the support
Uniform particle size
Particle size availability: 1.7, 3, 5, and 10 µm
Weak-cation exchange chemistry
Hydrophilic surface specially designed for elimination of non-specific bindings
High separation efficiency, selectivity and resolving power
High lot-to-lot reproducibility (link to below header)
High loading capacity (link to below header)
High recovery
High mechanical stability with pressure tolerance higher than 8,000 psi for 5 and 10 µm, and 10,000 psi for 1.7 and 3 µm
High pH stability: 2-12
Well suited for UPLC and regular HPLC systems
Complete selection for analytical, semi-preparative and preparative separations
Applications for proteins, especially antibodies

Chromatogram of a protein mixture separated by Antibodix NP5, 7.8×50mm

Conditions
Column:	Antibodix NP5 (5 µm, 7.8×50 mm)
Mobile phase:	A: 10 mM phosphate buffer, pH 6 B: A + 1.0 M NaCl
Gradient:	10-45%B (21 min)
Flow rate:	1.5 mL/min
Temperature:	Ambient
Injection volume:	10 µL
Sample:	1. Cytochrome C 2. Lysozyme 3. Ribonuclease A

Separation of MAb on Antibodix NP1.7 (1.7 µm, 4.6×50 mm)

Conditions
Column:	Antibodix NP1.7 (1.7 µm, 4.6×50 mm)
Mobile phase:	A. 10 mM phosphate buffer, pH 7.5 B. A + 100 mM NaCl
Gradient:	15-55% B (30 min)
Flow rate:	0.3 mL/min
Detector:	UV 214 nm
Temperature:	Ambient
Injection volume:	5 µL
Sample:	Monoclonal antibody (5 mg/mL)

High lot to lot reproducibility

Sample 1. Protein mixtures

Conditions
Column:	Antibodix NP10 (10 µm, 4.6×250 mm)
Mobile phase:	A: 10 mM phosphate buffer, pH 7.5 B: A + 100 mM NaCl
Gradient:	15-55%B (30 min)
Flow rate:	0.8 mL/min
Temperature:	Ambient
Detector:	UV 214 nm
Injection volume:	5 µL
Sample:	1. Cytochrome C (5 mg/mL) 2. Lysozyme (5 mg/mL) 3. Ribonuclease A (5 mg/mL)

Sample 2. Monoclonal antibodies

Conditions
Column:	Antibodix NP10 (10 µm, 4.6×250 mm)
Mobile phase:	A. 10 mM phosphate buffer, pH 7.5 B. A + 100 mM NaCl
Gradient:	15-55% B (30 min)
Flow rate:	0.8 mL/min
Temperature:	Ambient
Detector:	UV 214 nm
Injection volume:	5 µL
Sample:	Monoclonal antibody (5 mg/mL)

High Loading

Conditions
Column:	Antibodix NP10 (10 µm, 4.6×250 mm)
Mobile phase:	A: 10 mM phosphate buffer, pH 6 B: A + 1.0 M NaCl
Gradient:	10-100%B (25 min)
Flow rate:	0.8 mL/min
Temperature:	Ambient
Detector:	UV 214 nm
Sample:	1. Aprotinin (3.3 mg/mL) 2. Lysozyme (5.6 mg/mL) 3. Ribonuclease (3.0 mg/mL)

Antibodix WCX

NP1.7

NP3

NP5

NP10

Sepax Antibodix WCX-NP1.7

Part Number	Particle Size	Pore Size	ID×Length

602NP2-2001 ^[1]	1.7 µm	Non-Porous	2.0×10mm
602NP2-2001C ^[2]	1.7 µm	Non-Porous	2.0×10mm
602NP2-2103	1.7 µm	Non-Porous	2.1×30mm
602NP2-2105	1.7 µm	Non-Porous	2.1×50mm
602NP2P-2105 ^[3]	1.7 µm	Non-Porous	2.1×50mm
602NP2-2110	1.7 µm	Non-Porous	2.1×100mm
602NP2-3003	1.7 µm	Non-Porous	3.0×30mm
602NP2-3005	1.7 µm	Non-Porous	3.0×50mm
602NP2-3010	1.7 µm	Non-Porous	3.0×100mm
602NP2-4001 ^[1]	1.7 µm	Non-Porous	4.0×10mm
602NP2-4001C ^[2]	1.7 µm	Non-Porous	4.0×10mm
602NP2-4603	1.7 µm	Non-Porous	4.6×30mm
602NP2-4605	1.7 µm	Non-Porous	4.6×50mm
602NP2P-4605 ^[3]	1.7 µm	Non-Porous	4.6×50mm
602NP2-4610	1.7 µm	Non-Porous	4.6×100mm
602NP2-4615	1.7 µm	Non-Porous	4.6×150mm
602NP2-7805	1.7 µm	Non-Porous	7.8×50mm
602NP2-7807	1.7 µm	Non-Porous	7.8×75mm
602NP2-7810	1.7 µm	Non-Porous	7.8×100mm

[1]: Guard Cartridge only.
[2]: Guard Cartridge with holder (Part number ended with "C").

Precolumn Filters (Alternative choice for Guard Cartridge)
P/N#: 102000-P355 (2 µm PEEK frit), $65.00/ea
- for column with particle size 5 µm and 10 µm.
P/N#: 102000-P356 (0.5 µm PEEK frit), $65.00/ea
- for column with particle size 3 µm, or less than 3 µm.

PEEK Refill Frits (For Precolumn Filter)
P/N#: 102001-P355, 2 µm, 5 units/pk, $100/pk
P/N#: 102001-P356, 0.5 µm, 5 units/pk, $100/pk

[3] Column packed with PEEK tubing.