Proteomix® HIC columns are specially designed for high resolution and high efficiency separations of proteins, monoclonal antibodies (mAbs), antibody drug conjugates (ADCs), oligonucleotides and peptides via a hydrophobic interaction chromatography (HIC) mechanism. Utilizing proprietary surface technologies, Proteomix® HIC-NP resin is made of non-porous polystyrenedivinylbenzene (PS/DVB) beads with narrow-dispersed particle size distribution. As shown in figure below, the PS/DVB bead is modified with alkyl groups or aryl groups that provide hydrophobic interaction with analytes.
Proteomix® HIC-NP resin is highly rigid and mechanically stable. In comparison to silica-based HIC phase media, Proteomix® HIC-NP phases have advantages for biomolecule separations with a wide pH range (2-12) and high chemical stability. The nonporous structure, unique chemistry, and narrow particle distribution offer special selectivity, high-resolution separation of proteins such as mAb, ADC and related protein fragments, DNA and oligonucleotides. Proteomix® HIC-NP media is applicable at laboratory discovery, laboratory-scale purification and preparative chromatography for the production of several milligrams to grams of proteins. Sepax also provides polymethacrylate-based Generik MC and Polar MC HIC 30 µm media which offers similar characteristics to the Proteomix® HIC-NP but is designed for process-scale applications from grams to kilograms of proteins.
Hydrophobicity Order of the four HIC NP phase:
Ethyl<Propyl<Butyl<Phenyl
Protein Mixture on Four Proteomix® HIC NP5 Phases
Proteomix® HIC Technical Specifications
Resin Matrix
Spherical, highly cross-linked PS/DVB
Pore Size
Nonporous
Particle Size
1.7, 5 and 10 µm
Phase Structure
Ethyl, Propyl, Butyl, and Phenyl (5, 10 µm) Ethyl and Butyl (1.7 µm)
Separation Mechanism
Hydrophobic interaction
pH Stability
2-12
Operating Temperature
Up to 80 °C
Operating Pressure
6000 psi (5, 10 µm) 8000 psi (1.7 µm)
Mobile Phase Compatibility
Compatible with aqueous solution, a mixture of water and acetonitrile, acetone, methanol, or THF
Highest capacity and resolution
High protein recovery with intact biological activity
High stability and lot-to-lot reproducibility
High pressure and high temperature tolerance
Ideal for separation and analysis of hydrophobic proteins, monoclonal antibodies and their derivatives such as antibody drug conjugates, and organic molecules derivatized with polymer branches
[1] Guard Cartridge with holder. [2] Guard column [3] Sepax Protein standards for Proteomix HIC, 5 vials, 50 µl/vial [4] One-piece PEEK coupler, recommended to connect columns in series. [5] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [6] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [7] 0.5 µm PEEK frit for column with particle size 3 µm or less [8] For Precolumn Filter (0.5 µm) 5 units/pk
[1] Guard Cartridge with holder. [2] Sepax Protein standards for Proteomix HIC, 5 vials, 50 µl/vial [3] One-piece PEEK coupler, recommended to connect columns in series. [4] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [5] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [6] 0.5 µm PEEK frit for column with particle size 3 µm or less [7] For Precolumn Filter (0.5 µm) 5 units/pk
[1] Sepax Protein standards for Proteomix HIC, 5 vials, 50 µl/vial [2] One-piece PEEK coupler, recommended to connect columns in series. [3] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [4] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [5] 0.5 µm PEEK frit for column with particle size 3 µm or less [6] For Precolumn Filter (0.5 µm) 5 units/pk
[1] Sepax Protein standards for Proteomix HIC, 5 vials, 50 µl/vial [2] One-piece PEEK coupler, recommended to connect columns in series. [3] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [4] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [5] 0.5 µm PEEK frit for column with particle size 3 µm or less [6] For Precolumn Filter (0.5 µm) 5 units/pk
[1] Guard Cartridge with holder. [2] Guard column [3] Sepax Protein standards for Proteomix HIC, 5 vials, 50 µl/vial [4] One-piece PEEK coupler, recommended to connect columns in series. [5] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [6] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [7] 2 µm PEEK frit for column with particle size 5 µm and 10 µm. [8] Intended for use with column IDs ≥ 21.2 mm [9] For Precolumn Filter (2 µm) 5 units/pk [10] SS Refill Frits for 102020-21200, 5 units/pk
[1] Guard Cartridge with holder. [2] Sepax Protein standards for Proteomix HIC, 5 vials, 50 µl/vial [3] One-piece PEEK coupler, recommended to connect columns in series. [4] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [5] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [6] 2 µm PEEK frit for column with particle size 5 µm and 10 µm. [7] For Precolumn Filter (2 µm) 5 units/pk
[1] Guard Cartridge with holder. [2] Sepax Protein standards for Proteomix HIC, 5 vials, 50 µl/vial [3] One-piece PEEK coupler, recommended to connect columns in series. [4] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [5] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [6] 2 µm PEEK frit for column with particle size 5 µm and 10 µm. [7] For Precolumn Filter (2 µm) 5 units/pk
[1] Guard Cartridge with holder. [2] Sepax Protein standards for Proteomix HIC, 5 vials, 50 µl/vial [3] One-piece PEEK coupler, recommended to connect columns in series. [4] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [5] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [6] 2 µm PEEK frit for column with particle size 5 µm and 10 µm. [7] For Precolumn Filter (2 µm) 5 units/pk
