A proprietary surface coating technology was developed for coating the capillary columns. This novel surface synthesis has following advantages.
- In the process of synthesis, the polymer brushes are specifically bonded on the silica surface, which greatly simplifies the manufacturing process.
- The polymer film thickness can be tuned in the range from a few nanometers to a few hundreds of nanometers.
- The density of the polymer brushes can be well controlled.
- The coating is uniform.
- Polymer brushes can be readily functionized for various applications.
| Capillary column: |
PEG coated fused silica |
| Inner diameter: |
75, 50, and 25 µm |
| Coating: |
PEG thin film |
| Coating thickness: |
~ 5, 15, 25, and 50 nm |
| Application pH range: |
2 - 9.5 |
Separation of protein standards by a 75 µm I.D. PEG coated capillary column (N=1.33×105/m for cytochrome C).
Separation of protein standards by a 50 µm I.D. PEG coated capillary column with the same conditions as that in Figure 1 (N=1.26×106/m for cytochrome C).
- The PEG coating is neutral and hydrophilic
- Extremely high column-to-column reproducibility
- Negligible electro-osmotic flow
- Eliminated non-specific interactions with biological molecules
- Ideal for separations of proteins and other biological molecules
- Suitable for DNA separation and sequencing
EOF of PEG Coated Capillary
- PEG coating ~ 20 nm thick
- ~10% of uncoated capillary EOF
- µEOF < 1.1×10-6 cm2/vs for pH 2, 3, and 4
| Conditions | |
| Capillary: | 40×31.5 cm, 75 µm i.d. |
| Coating: | PEG ~20 nm thickness |
| Sample: | acetone (100 ppm) |
| Buffer: | 25 mM phosphate buffer at various pH |
| Applied voltage: | 15 kV |
| Injection: | 50 mbar, 2 s |
The unique coating technology makes uniform poly(ethylene glycol) thin film on the surface of the capillary inner wall. The very hydrophilic PEG coating with the thickness controlled in the range of 1¨C50 nm makes the capillary column ideal for protein separation. Such uniform stationary phases allow separations with high selectivity and high efficiency. Compared with the PAAm coating, the PEG coating is more stable in both acidic and basic conditions. The novel coating technology readily applies to very narrow capillary tube, such as 20 µm diameter. With the decrease of the diameter of the capillary column, the separation resolution and efficiency are greatly improved.
Separation of chiral compounds
SepaxCE-PEG
(Ltotal × LEffective) | ID | P/N | Price |
|---|
|
| 75×65 | 75 µm | 301275-7510 | $195.00 |
| 50×40 | 75 µm | 301275-5010 | $185.00 |
| 50×35 | 75 µm | 301275-5015 | $185.00 |
| 30×20 | 75 µm | 301275-3010 | $175.00 |
| |
| 75×65 | 50 µm | 301250-7510 | $195.00 |
| 50×40 | 50 µm | 301250-5010 | $185.00 |
| 50×35 | 50 µm | 301250-5015 | $185.00 |
| 30×20 | 50 µm | 301250-3010 | $175.00 |
| |
| 75×65 | 25 µm | 301225-7510 | $225.00 |
| 50×40 | 25 µm | 301225-5010 | $215.00 |
| 50×35 | 25 µm | 301225-5015 | $215.00 |
| 30×20 | 25 µm | 301225-3010 | $205.00 |
| |
| 75×65 | 15 µm | 301215-7510 | $255.00 |
| 50×40 | 15 µm | 301215-5010 | $245.00 |
| 50×35 | 15 µm | 301215-5015 | $245.00 |
| 30×20 | 15 µm | 301215-3010 | $235.00 |
|
