Hydrophobic Interaction Chromatographic (HIC) separation applies to all stages of biological sample purification process including capturing and polishing. Sepax manufactures two HIC products: Generik MC-HIC and Polar MC-HIC. Both Polar MC-HIC and Generik MC-HIC are made of polymethacrylate matrix, while Generik MC is more hydrophobic than Polar MC.
Generik MC30-HIC Butyl media is composed of polymethacrylate beads functionalized with butyl on the surface, as shown in Figure 1. This media has an average particle size of 30 µm and pore size of 800 Å. It has high physical and chemical stability and can tolerate high-pressure operation up to 100 bars which allows faster flow rate and shorter running time. These resins are specially designed for various stages of hydrophobic interaction chromatography separation needs, from laboratory discovery, pilot-scale purification to industrial process.
Generik MC30-HIC Butyl
70% (v/v) slurry in 20% ethanol
20 - 45 µm (average 30 µm)
Dynamic Binding Capacity*
45 ± 5 mg Lysozyme /mL
Up to 40 °C
Up to 100 bar
Mobile Phase Compatibility
Compatible with aqueous solution, a mixture of water and acetonitrile, acetone, or methanol. Typical buffers: phosphate, Tris, & acetate.
Linear Flow Rate
Up to 7200 cm/hour
*DBC was tested with lysozyme (1 mg/mL) in 25 mM sodium phosphate pH 7 + 2 M (NH4)2SO4 with 360 cm/h flow rate at 280 nm, 10% breakthrough.
Unique hydrophobic interaction
High accessible surface area and loading capacity
High chemical stability for low leaching
High physical stability for high-pressure operation
Capable of running high flow rate to speed up separation
Spherical particles with high mechanical strength
Wide pH tolerance (pH=2-14)
Available from 1 L to >100 L
Generik MC30-HIC Butyl media offers excellent high efficiency and recovery separation of bio-molecules such as mAb (monoclonal antibody), ADC (antibody drug conjugate) and related protein fragments, DNA and oligonucleotides.