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SRT - High Resolution Size Exclusion Chromatography Columns

Featured Characteristics

  • Highest pore volume, capacity and resolution
  • Widest selection of pore size from 100 to 2000 Å
  • High stability over low and high concentration salt
  • High lot-to-lot reproducibility
  • High protein recovery and maintaining biological activity
  • Negligible non-specific interactions
  • Ideal for separation and analysis of biological molecules: proteins, nucleic acids, oligonucleotides, peptides and virus
  • Ideal for separation and analysis of natural polymers, e.g. polysaccharides, synthetic polymers, and nanomaterials, e.g. nanoparticles

Separation of Protein and Peptide Mixture

Figure 1. Comparison of SRT SEC-150 and a similar pore size SEC column from Vendor T.

Figure 1
Column:7.8×300 mm, 5 µm
Mobile phase:150 mM PBS, pH 7
Flow rate:1.0 mL/min
Temperature:ambient (~23 °C)
Detection:UV 214nm
Injection:10 µL
Sample:1) Thyroglobulin, 670kD
2) BSA dimer, 132kD
3) BSA monomer, 66kD
4) Ribonuclease A, 13.7kD
5) poly-DL-alanine, 1-5 kD
6) Uracil, 120D

Figure 2. Comparison of SRT SEC-300 and a similar pore size SEC column from Vendor T.

Figure 2
Column:7.8×300 mm, 5 µm
Mobile phase:150 mM PBS, pH 7
Flow rate:1.0 mL/min
Temperature:ambient (~23 °C)
Detection:UV 214nm
Injection:10 µL
Sample:1) Thyroglobulin aggregate
2) Thyroglobulin, 670kD
3) γ-Globulin dimer
4) γ-Globulin, 158 kD
5) Ovalbumin, 44kD
6) Myoglobin, 17.6 kD
7) Poly-DL-alanine, 1-5 kD
8) Uracil, 120D
 
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