A proprietary surface coating technology was developed for coating the capillary columns. This novel surface synthesis has following advantages.
- In the process of synthesis, the polymer brushes are specifically bonded on the silica surface, which greatly simplifies the manufacturing process.
- The polymer film thickness can be tuned in the range from a few nanometers to a few hundreds of nanometers.
- The density of the polymer brushes can be well controlled.
- The coating is uniform.
- Polymer brushes can be readily functionized for various applications.
| Capillary column: |
PAAm coated fused silica |
| Inner diameter: |
75, 50, 25 and 15 µm |
| Coating: |
PAAm thin film |
| Coating thickness: |
~ 25 nm |
| Application pH range: |
2 - 8.5 |
- The novel coating technology readily applies to very narrow capillary tubes, e.g., 10 µm ID
- The PAAm coating is neutral and hydrophilic
- High column-to-column reproducibility
- Negligible electro-osmotic flow
- Eliminated non-specific interactions with biological molecules
- Ideal for separations of proteins and other biological molecules
- Suitable for DNA separation and sequencing
The unique coating technology makes uniform polyacrylamide thin film on the surface of the capillary inner wall. The very hydrophilic PAAm coating with the thickness of ~ 25 nm makes the capillary column ideal for protein separations. Such uniform stationary phases allow the separation to achieve high selectivity and high efficiency. A typical test electropherogram for a 75 µm diameter PAAm coated capillary column is shown in the above.
Sepax's novel surface technology can even coat very narrow capillary tubes, down to a few microns. One example shown here is coating a 20 µm inner diameter capillary column. With the decrease of the diameter of the capillary column, the separation resolution and efficiency are greatly improved.
SepaxCE-PAAm
(Ltotal × LEffective) | ID | P/N | Price |
|---|
|
| 75×65 | 75 µm | 301175-7510 | $195.00 |
| 50×40 | 75 µm | 301175-5010 | $185.00 |
| 50×35 | 75 µm | 301175-5015 | $185.00 |
| 30×20 | 75 µm | 301175-3010 | $175.00 |
| |
| 75×65 | 50 µm | 301150-7510 | $195.00 |
| 50×40 | 50 µm | 301150-5010 | $185.00 |
| 50×35 | 50 µm | 301150-5015 | $185.00 |
| 30×20 | 50 µm | 301150-3010 | $175.00 |
| |
| 75×65 | 25 µm | 301125-7510 | $225.00 |
| 50×40 | 25 µm | 301125-5010 | $215.00 |
| 50×35 | 25 µm | 301125-5015 | $215.00 |
| 30×20 | 25 µm | 301125-3010 | $205.00 |
| |
| 75×65 | 15 µm | 301115-7510 | $255.00 |
| 50×40 | 15 µm | 301115-5010 | $245.00 |
| 50×35 | 15 µm | 301115-5015 | $245.00 |
| 30×20 | 15 µm | 301115-3010 | $235.00 |
|
